Typical Testing Data/Standard Curve (for reference only)
(Mouse B7-1/CD80 PicoKine ELISA Kit standard curve )
Mouse B7-1/CD80 ELISA Kit | CD80 elisa kit
Mouse B7-1/CD80 PicoKine ELISA Kit
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Reactivity
Mouse
Synonyms
B7-1/CD80; Mouse B7-1/CD80 PicoKine ELISA Kit; T-lymphocyte activation antigen CD80; CD80 molecule; Activation B7-1 antigen; B7; BB1; CD80; CD80_HUMAN; CTLA-4 counter-receptor B7.1; CD80 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD80
Sequence Length
306
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse B7-1/CD80 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse B7-1/CD80 PicoKine ELISA Kit standard curve )
Related Product Information for CD80 elisa kit
Principle of the assay: mouse CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD80 has been precoated onto 96-well plates. Standards(NSO, D37-K245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD80 amount of sample captured in plate.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,051 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 antigen
NCBI Official Symbol
Cd80
NCBI Official Synonym Symbols
B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
NCBI Protein Information
T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen
UniProt Protein Name
T-lymphocyte activation antigen CD80
Protein Family
UniProt Gene Name
Cd80
UniProt Synonym Gene Names
B7; B7
UniProt Entry Name
CD80_MOUSE
Uniprot Description
CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
Protein type: Membrane protein, integral; Immunoglobulin superfamily
Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane
Molecular Function: protein binding
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation
Research Articles on CD80
Similar Products
Product Notes
The Mouse CD80 cd80 (Catalog #AAA175825) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA175825 ELISA Kit recognizes Mouse CD80. It is sometimes possible for the material contained within the vial of "B7-1/CD80, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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