Immunohistochemistry (IHC)
(Anti-MCM7 antibody, MBS175521, IHC(P)IHC(P): Human Lung Cancer Tissue )
Rabbit anti-Human MCM7 Polyclonal Antibody | anti-MCM7 antibody
Anti-MCM7 antibody
Immunohistochemistry (IHC)
(Anti-MCM7 antibody, MBS175521, IHC(P)IHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-MCM7 antibody, MBS175521, IHC(P)IHC(P): Human Lung Cancer Tissue )
Western Blot (WB)
(Anti-MCM7 antibody, MBS175521, Western blottingLane 1: COLO320 Cell LysateLane 2: SW620 Cell LysateLane 3: HELA Cell LysateLane 4: 22RVL Cell LysateLane 5: 293T Cell LysateLane 6: U937 Cell LysateLane 7: JURKAT Cell LysateLane 8: RAJI Cell Lysate)
Western Blot (WB)
(Anti-MCM7 antibody, MBS175521, Western blottingLane 1: COLO320 Cell LysateLane 2: SW620 Cell LysateLane 3: HELA Cell LysateLane 4: 22RVL Cell LysateLane 5: 293T Cell LysateLane 6: U937 Cell LysateLane 7: JURKAT Cell LysateLane 8: RAJI Cell Lysate)
Immunocytochemistry (ICC)
(Anti-MCM7 antibody, MBS175521, ICCICC: MCF-7 Cell )
Immunocytochemistry (ICC)
(Anti-MCM7 antibody, MBS175521, ICCICC: MCF-7 Cell )
Immunocytochemistry (ICC)
(Anti-MCM7 antibody, MBS175521, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-MCM7 antibody, MBS175521, ICCICC: HELA Cell )
Background: MCM7(Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al.(2008) found that MCM7 and the precursors of microRNAs(miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
MCM7: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. Belongs to the MCM family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.6.4.12; DNA replication; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q21.3-q22.1
Cellular Component: nucleoplasm; membrane; MCM complex; cytoplasm; chromatin; cytosol
Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; single-stranded DNA binding; ATP binding
Biological Process: DNA unwinding during replication; response to drug; cell proliferation; DNA replication initiation; regulation of phosphorylation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; response to DNA damage stimulus; G1/S transition of mitotic cell cycle
Research Articles on MCM7
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Product Notes
The MCM7 mcm7 (Catalog #AAA175521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM7 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCM7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the MCM7 mcm7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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