Western Blot (WB)
(Figure 1. Western blot analysis of GMPS using anti-GMPS antibody (MBS1753604).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human COLO320 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human THP-1 whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human K562 whole cell lysatesLane 9: rat PC-12 whole cell lysatesLane 10: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GMPS antigen affinity purified polyclonal antibody (Catalog # MBS1753604) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GMPS at approximately 77KD. The expected band size for GMPS is at 77KD. )
Rabbit GMPS Polyclonal Antibody | anti-GMPS antibody
Anti-GMPS Antibody
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of GMPS using anti-GMPS antibody (MBS1753604).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human COLO320 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human THP-1 whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human K562 whole cell lysatesLane 9: rat PC-12 whole cell lysatesLane 10: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GMPS antigen affinity purified polyclonal antibody (Catalog # MBS1753604) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GMPS at approximately 77KD. The expected band size for GMPS is at 77KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of GMPS using anti-GMPS antibody (MBS1753604).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human HL-60 whole cell lysatesLane 4: human COLO320 whole cell lysatesLane 5: human Raji whole cell lysatesLane 6: human THP-1 whole cell lysatesLane 7: human Jurkat whole cell lysatesLane 8: human K562 whole cell lysatesLane 9: rat PC-12 whole cell lysatesLane 10: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GMPS antigen affinity purified polyclonal antibody (Catalog # MBS1753604) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for GMPS at approximately 77KD. The expected band size for GMPS is at 77KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HELA cells using anti-GMPS antibody (MBS1753604).Overlay histogram showing HELA cells stained with MBS1753604 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GMPS Antibody (MBS1753604, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HELA cells using anti-GMPS antibody (MBS1753604).Overlay histogram showing HELA cells stained with MBS1753604 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GMPS Antibody (MBS1753604, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Pegram, L. D., Megonigal, M. D., Lange, B. J., Nowell, P. C., Rowley, J. D., Rappaport, E. F., Felix, C. A. t(3;11) translocation in treatment-related acute myeloid leukemia fuses MLL with the GMPS (guanosine 5-prime monophosphate synthetase) gene. Blood 96: 4360-4362, 2000.
NCBI and Uniprot Product Information
NCBI Description
In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]
Uniprot Description
GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Oncoprotein; Ligase; Nucleotide Metabolism - purine; EC 6.3.5.2
Chromosomal Location of Human Ortholog: 3q24
Cellular Component: cytoplasm; cytosol
Molecular Function: pyrophosphatase activity; enzyme binding; GMP synthase activity; GMP synthase (glutamine-hydrolyzing) activity; ATP binding
Biological Process: response to drug; purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; glutamine metabolic process; purine base metabolic process
Disease: Leukemia, Acute Myeloid
Research Articles on GMPS
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Product Notes
The GMPS gmps (Catalog #AAA1753604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GMPS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GMPS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.1-0.25ug/ml|Human, Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the GMPS gmps for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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