Mouse anti-Human GMPS / GMP Synthase Monoclonal Antibody | anti-GMPS antibody

Mouse Monoclonal [clone 1D10] (IgG1,k) to Human GMPS / GMP Synthase

Cat. Num. AAA246611

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: GMPS / GMP Synthase; Monoclonal Antibody; Mouse Monoclonal [clone 1D10] (IgG1; k) to Human GMPS / GMP Synthase; Anti-GMPS / GMP Synthase Antibody (clone 1D10) IHC-plus; GMPS; GMP synthetase; Glutamine amidotransferase; GMP synthase; MLL/GMPS fusion protein; Human GMPS; GMP Synthase; anti-GMPS antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1D10
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.48 mg/ml (varies by lot)
• Sequence Length: 693

• Applicable Applications for anti-GMPS antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western blot performed with the recombinant protein used as the immunogen as the protein source. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: GMPS (NP_003866, 108 a.a. ~ 216 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: GMPS / GMP Synthase antibody was raised against gMPS (NP_003866, 108 a.a. ~ 216 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-GMPS antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GMPS antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged GMPS is approximately 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-GMPS antibody

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP.

NCBI and Uniprot Product Information

• NCBI GI #: 4504035
• NCBI GeneID: 8833
• NCBI Accession #: NP_003866.1
• NCBI GenBank Nucleotide #: NM_003875.2
• UniProt Accession #: P49915; A8K639; B4DXV7; F8W720
• Molecular Weight: 65,929 Da
• NCBI Official Full Name: GMP synthase
• NCBI Official Synonym Full Names: guanine monphosphate synthase
• NCBI Official Symbol: GMPS
• NCBI Protein Information: GMP synthase [glutamine-hydrolyzing]; GMP synthase [glutamine-hydrolyzing]; GMP synthetase; MLL/GMPS fusion protein; glutamine amidotransferase; guanine monphosphate synthetase; guanosine 5'-monophosphate synthase
• UniProt Protein Name: GMP synthase [glutamine-hydrolyzing]
• UniProt Gene Name: GMPS
• UniProt Entry Name: GUAA_HUMAN

NCBI Description

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]

Uniprot Description

GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.

Protein type: Oncoprotein; Ligase; EC 6.3.5.2; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: cytoplasm; cytosol

Molecular Function: pyrophosphatase activity; enzyme binding; GMP synthase activity; GMP synthase (glutamine-hydrolyzing) activity; ATP binding

Biological Process: response to drug; purine ribonucleoside monophosphate biosynthetic process; purine base biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; glutamine metabolic process; purine base metabolic process

Disease: Leukemia, Acute Myeloid