Western Blot (WB)
(Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (MBS1753501).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Sw620 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # MBS1753501) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD. )
Rabbit anti-Human, Mouse Utrophin/UTRN Polyclonal Antibody | anti-UTRN antibody
Anti-Utrophin/UTRN Antibody
ICC/IF: 5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (MBS1753501).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Sw620 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # MBS1753501) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Utrophin/UTRN using anti-Utrophin/UTRN antibody (MBS1753501).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Sw620 whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: mouse lung tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Utrophin/UTRN antigen affinity purified polyclonal antibody (Catalog # MBS1753501) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Utrophin/UTRN at approximately 394KD. The expected band size for Utrophin/UTRN is at 394KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of Utrophin/UTRN using anti- Utrophin/UTRN antibody (MBS1753501).Utrophin/UTRN was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Utrophin/UTRN Antibody (MBS1753501) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of Utrophin/UTRN using anti- Utrophin/UTRN antibody (MBS1753501).Utrophin/UTRN was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Utrophin/UTRN Antibody (MBS1753501) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
2. Blake, D. J., Schofield, J. N., Zuellig, R. A., Gorecki, D. C., Phelps, S. R., Barnard, E. A., Edwards, Y. H., Davies, K. E. G-utrophin, the autosomal homologue of dystrophin Dp116, is expressed in sensory ganglia and brain. Proc. Nat. Acad. Sci. 92: 3697-3701, 1995.
3. Buckle, V. J., Guenet, J. L., Simon-Chazottes, D., Love, D. R., Davies, K. E. Localisation of a dystrophin-related autosomal gene to 6q24 in man, and to mouse chromosome 10 in the region of the dystrophia muscularis (dy) locus. Hum. Genet. 85: 324-326, 1990.
NCBI and Uniprot Product Information
NCBI Description
This gene shares both structural and functional similarities with the dystrophin gene. It contains an actin-binding N-terminus, a triple coiled-coil repeat central region, and a C-terminus that consists of protein-protein interaction motifs which interact with dystroglycan protein components. The protein encoded by this gene is located at the neuromuscular synapse and myotendinous junctions, where it participates in post-synaptic membrane maintenance and acetylcholine receptor clustering. Mouse studies suggest that this gene may serve as a functional substitute for the dystrophin gene and therefore, may serve as a potential therapeutic alternative to muscular dystrophy which is caused by mutations in the dystrophin gene. Alternative splicing of the utrophin gene has been described; however, the full-length nature of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a role in anchoring the cytoskeleton to the plasma membrane.
Research Articles on UTRN
Similar Products
Product Notes
The UTRN utrn (Catalog #AAA1753501) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Utrophin/UTRN Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Utrophin/UTRN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse| ICC/IF: 5ug/ml|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the UTRN utrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Utrophin/UTRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
