Rabbit anti-Mouse, Rat Caspase-9/Casp9 Polyclonal Antibody | anti-Casp9 antibody

Anti-Caspase-9/Casp9 Antibody

Cat. Num. AAA1753275

• Gene Names: Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Caspase-9/Casp9; Polyclonal Antibody; Anti-Caspase-9/Casp9 Antibody; Casp9; Mch6; Caspase-9; CASP-9; EC 3. 4. 22. 62; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6 [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]; caspase 9; anti-Casp9 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for Caspase-9/Casp9 detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-Casp9 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Mouse|; IHC-P: 2-5ug/ml|Mouse, Rat|; ICC/IF: 5ug/ml|Mouse|; FC/FACS/FCM: 1-3ug/1x106 cells|Mouse|; Direct ELISA: 0.1-0.5ug/ml|Mouse|
• Immunogen: E Coli-derived mouse Casp9 recombinant protein (Position: E19-S454).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-Casp9 antibody

CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

References

1. Cingeetham A, et al. Association of caspase9 promoter polymorphisms with the susceptibility of AML in south Indian subjects. Tumour Biol, 2014 Sep.
2. Xu D, et al. Apoptotic block in colon cancer cells may be rectified by lentivirus mediated overexpression of caspase-9. Acta Gastroenterol Belg, 2013 Dec.

NCBI and Uniprot Product Information

• NCBI GI #: 31560479
• NCBI GeneID: 12371
• NCBI Accession #: NP_056548.2
• NCBI GenBank Nucleotide #: NM_015733.5
• UniProt Accession #: Q8C3Q9
• Molecular Weight: 49,979 Da
• NCBI Official Full Name: caspase-9 isoform 1
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: Casp9
• NCBI Official Synonym Symbols: Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: Casp9
• UniProt Synonym Gene Names: Mch6; CASP-9; APAF-3; ICE-LAP6

NCBI Description

This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) ().