Mouse anti-Human CD146/MCAM Monoclonal Antibody | anti-MCAM antibody
Anti-CD146/MCAM Antibody (Monoclonal, 4C12)
Gene Names
MCAM; CD146; MUC18; HEMCAM; METCAM; MelCAM
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
CD146/MCAM; Monoclonal Antibody; Anti-CD146/MCAM Antibody (Monoclonal; 4C12); A32 antigen antibody; CD 146 antibody; CD146 antibody; CD146 antigen antibody; Cell surface glycoprotein MUC18 antibody; Cell surface glycoprotein P1H12 antibody; Gicerin antibody; MCAM antibody; Melanoma adhesion molecule antibody; Melanoma associated antigen A32 antibody; Melanoma associated antigen MUC18 antibody; Melanoma associated glycoprotein MUC18 antibody; Melanoma cell adhesion molecule antibody; Melanoma-associated antigen A32 antibody; Melanoma-associated antigen MUC18 antibody; MelCAM antibody; MUC 18 antibody; MUC18 antibody; MUC18_HUMAN antibody; S endo 1 antibody; S endo 1 endothelial associated antigen antibody; S-endo 1 endothelial-associated antigen antibody; Sendo 1 endothelial associated antigen antibody; Sendo1 antibody; anti-MCAM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4C12
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
646
Applicable Applications for anti-MCAM antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
WB: 0.1-0.5ug/ml (Human)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
IHC-F: 0.5-1ug/ml (Human)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
IHC-F: 0.5-1ug/ml (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-MCAM antibody
Description: Mouse IgG monoclonal antibody for CD146/MCAM detection. Tested with WB, IHC-P, IHC-F in Human.
Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
Background: CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
References
1. Bardin, N., Anfosso, F., Masse, J.-M., Cramer, E., Sabatier, F., Le Bivic, A., Sampol, J., Dignat-George, F.Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion.Blood 98: 3677-3684, 2001.
2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999.
3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.
2. Kuske, M. D. A., Johnson, J. P.Assignment of the human melanoma cell adhesion molecule gene (MCAM) to chromosome 11 band q23.3 by radiation hybrid mapping.Cytogenet. Cell Genet. 87: 258 only, 1999.
3. Taira, E., Nagino, T., Taniura, H., Takaha, N., Kim, C.-H., Kuo, C.-H., Li, B.-S., Higuchi, H., Miki, N.Expression and functional analysis of a novel isoform of gicerin, an immunoglobulin superfamily cell adhesion molecule.J. Biol. Chem. 270: 28681-28687, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18; HEMCAM; METCAM; MelCAM
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
Uniprot Description
MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane
Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
Research Articles on MCAM
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Product Notes
The MCAM mcam (Catalog #AAA1753227) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD146/MCAM Antibody (Monoclonal, 4C12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD146/MCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin. WB: 0.1-0.5ug/ml (Human) IHC-P (Embedded Section): 0.5-1ug/ml (Human) IHC-F: 0.5-1ug/ml (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the MCAM mcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD146/MCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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