Mouse TFF3 ELISA Kit | TFF3 elisa kit

Mouse TFF3 PicoKine ELISA Kit

Cat. Num. AAA1751566

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Mouse
• Synonyms: TFF3; Mouse TFF3 PicoKine ELISA Kit; Mouse TFF3; TFF3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse STC2
• Sequence Length: 94

• Samples: Cell Culture Supernates, Serum And Plasma(Heparin)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFF3 elisa kit

Principle of the Assay: The Picokine Mouse STC2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse STC2 with a 96-well strip plate that is pre-coated with antibody specific for STC2. The detection antibody is a biotinylated antibody specific for STC2. The capture antibody is A monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse STC2 with immunogen: Expression system for standard: NSO, Immunogen sequence: T25-R296. The kit is analytically validated with ready to use reagents. To measure Mouse STC2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse STC2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse STC2 in the sample!!Background/Introduction: Stanniocalcin-2 is a protein that in humans is encoded by the STC2 gene. It is mapped to 11; 11 A4. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its Cterminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre-and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers.

NCBI and Uniprot Product Information

• NCBI GI #: 281485608
• NCBI GeneID: 7033
• NCBI Accession #: NP_003217.3
• NCBI GenBank Nucleotide #: NM_003226.3
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3
• Protein Family: Trefoil factor

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]