Human TFF3 ELISA Kit | TFF3 elisa kit

Human TFF3 PicoKine ELISA Kit

Cat. Num. AAA177316

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Human
• Synonyms: TFF3; Human TFF3 PicoKine ELISA Kit; Trefoil factor 3; trefoil factor 3 (intestinal); hITF; hP1.B; Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; Polypeptide P1.B; TFF3_HUMAN; TFI; TREFOIL; Trefoil factor (intestinal); TFF3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TFF3
• Sequence Length: 80

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TFF3 PicoKine ELISA Kit standard curve )

Related Product Information for TFF3 elisa kit

Principle of the assay: human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards(E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF3 amount of sample captured in plate.
Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

NCBI and Uniprot Product Information

• NCBI GI #: 585328
• NCBI GeneID: 7033
• UniProt Accession #: Q07654; Q96NX0; Q9UDA5; E9PBB5
• Molecular Weight: 10,181 Da
• NCBI Official Full Name: Trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3 (intestinal)
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3; polypeptide P1.B
• UniProt Protein Name: Trefoil factor 3
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI; hITF; hP1.B
• UniProt Entry Name: TFF3_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response