FGF7/KGF ELISA Kit | FGF7 elisa kit

Monkey primate FGF7/KGF PicoKine ELISA Kit

Cat. Num. AAA1751077

• Gene Names: FGF7; KGF; HBGF-7
• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: FGF7/KGF; Monkey primate FGF7/KGF PicoKine ELISA Kit; Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF; FGF7 elisa kit

• Reactivity: Monkey; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Monkey FGF7
• Sequence Length: 97

• Applicable Applications for FGF7 elisa kit: ELISA (EIA)
• Samples: Cell culture supernatants, serum and plasma (heparin, edta, citrate)
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <4 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF7 elisa kit

Principle of the Assay: The Picokine™ Monkey FGF7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Monkey FGF7 with a 96-well strip plate that is pre-coated with antibody specific for FGF7. The detection antibody is a biotinylated antibody specific for FGF7. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Monkey FGF7 with immunogen: Expression system for standard: E.coli; Immunogen sequence: C32-T194. The kit is analytically validated with ready-to-use reagents. To measure Monkey FGF7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Monkey FGF7 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Monkey FGF7 in the sample.

Background/Introduction: Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of monkey primate and monkey primate homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.

Product Categories/Family for FGF7 elisa kit

Signal Transduction; Growth Factors/Hormones; FGF; Cancer; Growth Factors; Developmental Biology; Organogenesis; Excretory System Development; Kidney Development; Kits/Lysates/Other; ELISA Kits; growth factors and Hormones ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q96FG5; H0YNY5
• Molecular Weight: 22509 MW
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: fibroblast growth factor 7
• UniProt Protein Name: Fibroblast growth factor 7
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF; FGF-7; HBGF-7

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.