Human Mitogen-activated protein kinase kinase kinase 7 (Map3k7) ELISA Kit | Map3k7 elisa kit

Human Mitogen-activated protein kinase kinase kinase 7 (Map3k7) ELISA Kit

Cat. Num. AAA167491

• Gene Names: MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
• Reactivity: Human
• Synonyms: Mitogen-activated protein kinase kinase kinase 7 (Map3k7); Human Mitogen-activated protein kinase kinase kinase 7 (Map3k7) ELISA Kit; Map3k7 elisa kit

• Reactivity: Human
• Sequence Length: 491

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 25ng/L - 7000ng/L
• Sensitivity: 12.37ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Map3k7 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Mitogen-activated Protein Kinase Kinase Kinase 7 (also known as Map3k7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Map3k7 antibody. Map3k7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Map3k7 Antibody is added and binds to Map3k7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Map3k7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Map3k7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 21735566
• NCBI GeneID: 6885
• NCBI Accession #: NP_663306.1
• NCBI GenBank Nucleotide #: NM_145333.2
• UniProt Accession #: O43318; O43317; O43319; Q5TDN2; Q5TDN3; Q5TDT7; Q9NTR3; Q9NZ70; B2RE27; E1P523
• Molecular Weight: 64,230 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform D
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: CSCF; FMD2; TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.