Human insulin-like growth factors 1 receptor, IGF-1R ELISA Kit | IGF-1R elisa kit

Human insulin-like growth factors 1 receptor, IGF-1R ELISA Kit

Cat. Num. AAA161469

• Gene Names: Igf1r; hyft; CD221; IGF-1R; D930020L01; A330103N21Rik
• Reactivity: Human
• Synonyms: insulin-like growth factors 1 receptor; IGF-1R; Human insulin-like growth factors 1 receptor; IGF-1R ELISA Kit; IGF-1R elisa kit

• Reactivity: Human
• Sequence Length: 1369

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.3ng/ml - 90ng/ml
• Sensitivity: 0.13ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF-1R elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Insulin-like Growth Factors 1 Receptor (also known as IGF-1R) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human IGF-1R antibody. IGF-1R present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human IGF-1R Antibody is added and binds to IGF-1R in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IGF-1R antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human IGF-1R. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 112983656
• NCBI GeneID: 16001
• NCBI Accession #: NP_034643.2
• NCBI GenBank Nucleotide #: NM_010513.2
• UniProt Accession #: Q60751; O70438; Q62123
• Molecular Weight: 155,788 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor I receptor
• NCBI Official Symbol: Igf1r
• NCBI Official Synonym Symbols: hyft; CD221; IGF-1R; D930020L01; A330103N21Rik
• NCBI Protein Information: insulin-like growth factor 1 receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• UniProt Gene Name: Igf1r
• UniProt Synonym Gene Names: IGF-I receptor

Uniprot Description

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R (). When present in a hybrid receptor with INSR, binds IGF1 ().