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Typical Testing Data/Standard Curve (for reference only)

Human Early activation antigen CD69, CD69 ELISA Kit | CD69 elisa kit

Human Early activation antigen CD69, CD69 ELISA Kit

Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Synonyms
Early activation antigen CD69; CD69; Human Early activation antigen CD69; CD69 ELISA Kit; CD69 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
199
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.05-20ng/ml
Sensitivity
0.017ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CD69 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Early activation antigen CD69 (also known as CD69) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD69 antibody. CD69 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD69 Antibody is added and binds to CD69 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD69 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD69. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
969
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN

NCBI Description

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Subunit structure: Homodimer; disulfide-linked. Ref.6 Ref.7 Ref.8

Subcellular location: Membrane; Single-pass type II membrane protein.

Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.

Developmental stage: Earliest inducible cell surface glycoprotein acquired during lymphoid activation.

Induction: By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.

Post-translational modification: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.

Sequence similarities: Contains 1 C-type lectin domain.

Research Articles on CD69

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Product Notes

The Human CD69 cd69 (Catalog #AAA1606136) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1606136 ELISA Kit recognizes Human CD69. It is sometimes possible for the material contained within the vial of "Early activation antigen CD69, CD69, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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