Mouse Toll Like Receptor 4 (TLR4) CLIA Kit | TLR4 clia kit

Mouse Toll Like Receptor 4 (TLR4) CLIA Kit

Cat. Num. AAA2708839

• Gene Names: Tlr4; Lps; Ly87; Ran/M1; Rasl2-8
• Reactivity: Mouse
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Toll Like Receptor 4 (TLR4); Mouse Toll Like Receptor 4 (TLR4) CLIA Kit; Toll Like Receptor 4; CD284; TOLL; HToll; TLR4 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Toll Like Receptor 4 (TLR4).; No significant cross-reactivity or interference between Toll Like Receptor 4 (TLR4) and analogues was observed.
• Sequence Length: 835

• Applicable Applications for TLR4 clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 13.7-10,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 5.5pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Toll Like Receptor 4 (TLR4) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 4 (TLR4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Toll Like Receptor 4 (TLR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Toll Like Receptor 4 (TLR4) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 10946594
• NCBI GeneID: 21898
• NCBI Accession #: NP_067272.1
• NCBI GenBank Nucleotide #: NP_067272.1
• UniProt Accession #: Q9QUK6
• NCBI Official Full Name: toll-like receptor 4
• NCBI Official Synonym Full Names: toll-like receptor 4
• NCBI Official Symbol: Tlr4
• NCBI Official Synonym Symbols: Lps; Ly87; Ran/M1; Rasl2-8
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr4
• UniProt Synonym Gene Names: Lps
• UniProt Entry Name: TLR4_MOUSE

NCBI Description

This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]