Human Early activation antigen CD69 ELISA Kit | CD69 elisa kit

Human Early activation antigen CD69 ELISA Kit

Cat. Num. AAA761041

• Gene Names: CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• Reactivity: Human
• Synonyms: Early activation antigen CD69; Human Early activation antigen CD69 ELISA Kit; CD69 (Early activation antigen CD69)/CLEC2C/EA-1/Leu23/MLR-3/p60/GP32/28/AIM/BL-AC/P26/EA1/CD69 antigen/Activation inducer molecule/activation inducer molecule (AIM/CD69)/CD69 molecule/C-type lectin domain family 2 member C/early activation antigen CD69/early lymphocyte activation antigen/Early T-cell activation antigen p60/Leukocyte surface antigen Leu-23; CD69 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD59. No significant cross-reactivity or interference between CD59 and analogues was observed.
• Sequence Length: 199

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD59 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD59 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD69 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD59 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD59 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD59 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD59 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502681
• NCBI GeneID: 969
• NCBI Accession #: NP_001772.1
• NCBI GenBank Nucleotide #: NM_001781.2
• Molecular Weight: 22,559 Da
• NCBI Official Full Name: early activation antigen CD69
• NCBI Official Synonym Full Names: CD69 molecule
• NCBI Official Symbol: CD69
• NCBI Official Synonym Symbols: AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• NCBI Protein Information: early activation antigen CD69
• UniProt Protein Name: Early activation antigen CD69
• Protein Family: Early activation antigen
• UniProt Gene Name: CD69
• UniProt Synonym Gene Names: CLEC2C; AIM
• UniProt Entry Name: CD69_HUMAN

NCBI Description

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.