Typical Testing Data/Standard Curve (for reference only)
Rat Myosin Light Chain 1,3, Skeletal Muscle Isoform ELISA Kit | MYL1 elisa kit
Rat Myosin Light Chain 1,3, Skeletal Muscle Isoform ELISA Kit
Gene Names
MYL1; MLC1F; MLC3F
Reactivity
Rat
Synonyms
Myosin Light Chain 1; 3; Skeletal Muscle Isoform; Rat Myosin Light Chain 1; Skeletal Muscle Isoform ELISA Kit; MYL1 elisa kit
Reactivity
Rat
Sequence Length
194
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
6ng/L-1500ng/L
Sensitivity
3.03ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MYL1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Myocyte-specific Enhancer Factor 2A (also known as MEF2A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MEF2A antibody. MEF2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MEF2A Antibody is added and binds to MEF2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MEF2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MEF2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MEF2A antibody. MEF2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MEF2A Antibody is added and binds to MEF2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MEF2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MEF2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myosin light chain 1/3, skeletal muscle isoform isoform 1f
NCBI Official Synonym Full Names
myosin light chain 1
NCBI Official Symbol
MYL1
NCBI Official Synonym Symbols
MLC1F; MLC3F
NCBI Protein Information
myosin light chain 1/3, skeletal muscle isoform
UniProt Protein Name
Myosin light chain 1/3, skeletal muscle isoform
Protein Family
UniProt Gene Name
MYL1
UniProt Synonym Gene Names
MLC1/MLC3; MLC1F/MLC3F
UniProt Entry Name
MYL1_HUMAN
NCBI Description
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MYL1: Regulatory light chain of myosin. Does not bind calcium. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Contractile; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2q33-q34
Cellular Component: sarcomere; myofibril; muscle myosin complex; cytosol
Molecular Function: structural constituent of muscle; calcium ion binding
Biological Process: muscle contraction; muscle filament sliding; cardiac muscle contraction
Research Articles on MYL1
Similar Products
Product Notes
The Rat MYL1 myl1 (Catalog #AAA1603454) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603454 ELISA Kit recognizes Rat MYL1. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 1,3, Skeletal Muscle Isoform, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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