Typical Testing Data/Standard Curve (for reference only)
Rat Intercellular Adhesion Molecule 1, sICAM-1 ELISA Kit | sICAM-1 elisa kit
Rat Soluble Intercellular Adhesion Molecule 1, sICAM-1 ELISA Kit
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Rat
Synonyms
Intercellular Adhesion Molecule 1; sICAM-1; Rat Soluble Intercellular Adhesion Molecule 1; sICAM-1 ELISA Kit; sICAM-1 elisa kit
Reactivity
Rat
Sequence Length
38
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 20ng/ml
Sensitivity
0.026ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for sICAM-1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Soluble Intercellular Adhesion Molecule 1 (also known as sICAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat sICAM-1 antibody. sICAM-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat sICAM-1 Antibody is added and binds to sICAM-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated sICAM-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat sICAM-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat sICAM-1 antibody. sICAM-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat sICAM-1 Antibody is added and binds to sICAM-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated sICAM-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat sICAM-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,825 Da
NCBI Official Full Name
sICAM-1, partial
NCBI Official Synonym Full Names
intercellular adhesion molecule 1
NCBI Official Symbol
ICAM1
NCBI Official Synonym Symbols
BB2; CD54; P3.58
NCBI Protein Information
intercellular adhesion molecule 1
UniProt Protein Name
Intercellular adhesion molecule 1
UniProt Gene Name
ICAM1
UniProt Synonym Gene Names
ICAM-1
NCBI Description
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
Research Articles on sICAM-1
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Product Notes
The Rat sICAM-1 icam1 (Catalog #AAA160245) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA160245 ELISA Kit recognizes Rat sICAM-1. It is sometimes possible for the material contained within the vial of "Intercellular Adhesion Molecule 1, sICAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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