Mouse Complement Factor I (CFI) ELISA Kit | CFI elisa kit

Mouse Complement Factor I (CFI) ELISA Kit

Cat. Num. AAA160215

• Reactivity: Mouse
• Synonyms: Complement Factor I (CFI); Mouse Complement Factor I (CFI) ELISA Kit; CFI elisa kit

• Reactivity: Mouse
• Sequence Length: 603

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 2ng/ml - 600ng/ml
• Sensitivity: 1.05ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFI elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Complement Factor I (also known as CFI) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse CFI antibody. CFI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CFI Antibody is added and binds to CFI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CFI antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CFI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 110347406
• NCBI GeneID: 12630
• NCBI Accession #: NP_031712.2
• NCBI GenBank Nucleotide #: NM_007686.3
• UniProt Accession #: Q61129; Q9WU07; B2RWX8
• Molecular Weight: 67,261 Da
• NCBI Official Full Name: complement factor I isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component factor i
• NCBI Official Symbol: Cfi
• NCBI Protein Information: complement factor I
• UniProt Protein Name: Complement factor I
• Protein Family: Complement factor
• UniProt Gene Name: Cfi
• UniProt Synonym Gene Names: If

NCBI Description

This gene encodes a serine protease that plays an important role in the classical and alternative complement pathways where it cleaves C4b and C3b components of C3 and C5 convertases. The encoded preproprotein undergoes proteolytic processing to generate an active, disulfide-linked heterodimeric enzyme comprised of heavy and light chains. [provided by RefSeq, Jul 2016]

Uniprot Description

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.