Human C-C motif chemokine 22 (CCL22) ELISA Kit | CCL22 elisa kit

Human C-C motif chemokine 22 (CCL22) ELISA Kit

Cat. Num. AAA1600014

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Reactivity: Human
• Synonyms: C-C motif chemokine 22 (CCL22); Human C-C motif chemokine 22 (CCL22) ELISA Kit; CCL22 elisa kit

• Reactivity: Human
• Sequence Length: 93

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.03ng/ml - 10ng/ml
• Sensitivity: 0.01ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL22 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human C-C Motif Chemokine 22 (also known as CCL22) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CCL22 antibody. CCL22 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CCL22 Antibody is added and binds to CCL22 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCL22 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CCL22. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 22538804
• NCBI GeneID: 6367
• NCBI Accession #: NP_002981.2
• NCBI GenBank Nucleotide #: NM_002990.4
• UniProt Accession #: O00626; A0N0Q6; B2R4W2
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: C-C motif chemokine 22
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]

Uniprot Description

May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.