CANT1 recombinant protein

Recombinant Human CANT1 protein

Cat. Num. AAA1562269

• Gene Names: CANT1; DBQD; SCAN1; SHAPY; SCAN-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CANT1; Recombinant Human CANT1 protein; DBQD Protein; Human; SCAN-1 Protein; SCAN1 Protein; SHAPY Protein; CANT1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human CANT1 (Ile107-Phe400) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for CANT1 recombinant protein

CANT1(calcium activated nucleotidase 1) belongs to the apyrase family. Apyrase is a calcium-activated plasma membrane-bound enzyme (magnesium can also activate it) (EC 3.6.1.5) that catalyses the hydrolysis of ATP to yield AMP and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP: ADP and another with no selectivity. It can also act on ADP and other nucleoside triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi. The salivary apyrases of blood-feeding arthropods are nucleotide hydrolysing enzymes are implicated in the inhibition of host platelet aggregation through the hydrolysis of extracellular adenosine diphosphate. CANT1 functions as a calcium-dependent nucleotidase with a preference for UDP. Defects in CANT1 are the cause of desbuquois dysplasia.

Product Categories/Family for CANT1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 229577440
• NCBI GeneID: 124583
• NCBI Accession #: NP_001153244.1
• NCBI GenBank Nucleotide #: NM_001159772.1
• UniProt Accession #: Q8WVQ1; Q7Z2J7; Q8NG05; Q8NHP0; Q9BSD5; B4DJ54
• Molecular Weight: 38,946 Da
• NCBI Official Full Name: soluble calcium-activated nucleotidase 1
• NCBI Official Synonym Full Names: calcium activated nucleotidase 1
• NCBI Official Symbol: CANT1
• NCBI Official Synonym Symbols: DBQD; SCAN1; SHAPY; SCAN-1
• NCBI Protein Information: soluble calcium-activated nucleotidase 1; Ca2+-dependent endoplasmic reticulum nucleoside diphosphatase; apyrase homolog; micromelic dwarfism with vertebral and metaphyseal abnormalities and advanced carpotarsal ossification; putative MAPK-activating prot
• UniProt Protein Name: Soluble calcium-activated nucleotidase 1
• Protein Family: Soluble calcium-activated nucleotidase
• UniProt Gene Name: CANT1
• UniProt Synonym Gene Names: SHAPY; SCAN-1
• UniProt Entry Name: CANT1_HUMAN

NCBI Description

This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

CANT1: Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis. Defects in CANT1 are the cause of Desbuquois dysplasia (DBQD). A chondrodysplasia characterized by severe prenatal and postnatal growth retardation (less than -5 SD), joint laxity, short extremities, progressive scoliosis, round face, midface hypoplasia, prominent bulging eyes. The main radiologic features are short long bones with metaphyseal splay, a 'Swedish key' appearance of the proximal femur (exaggerated trochanter), and advance carpal and tarsal bone age. Two forms of Desbuquois dysplasia are distinguished on the basis of the presence (type 1) or absence (type 2) of characteristic hand anomalies: an extra ossification center distal to the second metacarpal, delta phalanx, bifid distal thumb phalanx, and phalangeal dislocations. Belongs to the apyrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Endoplasmic reticulum; Membrane protein, integral; EC 3.6.1.6

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: signal transducer activity; calcium ion binding; uridine-diphosphatase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; proteoglycan biosynthetic process; signal transduction

Disease: Desbuquois Dysplasia 1