Rabbit anti-Human HNRPL Polyclonal Antibody | anti-HNRPL antibody

HNRPL, CT (Heterogeneous Nuclear Ribonucleoprotein L, hnRNP L, P/OKcl.14, HNRNPL) (MaxLight 750)

Cat. Num. AAA6481050

• Gene Names: HNRNPL; HNRPL; hnRNP-L; P/OKcl.14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: HNRPL; Polyclonal Antibody; CT (Heterogeneous Nuclear Ribonucleoprotein L; hnRNP L; P/OKcl.14; HNRNPL) (MaxLight 750); anti-HNRPL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HNRPL.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-HNRPL antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 461-490 in the C-terminal region of human HNRPL.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HepG2 cell line lysates (35ug/lane) using MBS631323 (2ug/ml). HNRPL (arrow) was detected usingMBS631323.)

Western Blot (WB)

(Western Blot analysis of HNRPL (arrow) usingMBS631323. Lane 1: Nontransfected 293 cell lysates (2ug/lane) Lane 2: transfected 293 cell lysates (2ug/lane) with the HNRPL gene)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma usingMBS631323, followed by IgG (HRP) secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Product Categories/Family for anti-HNRPL antibody

Antibodies; Nuclear Proteins

References

1. Hahm, B., et.al., FEBS Lett. 425(3), 401-406 (1998). 2. Funke, B., et.al., Nucleic Acids Res. 24(19), 3821-3828 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 52632383
• NCBI GeneID: 3191
• NCBI Accession #: NP_001524
• NCBI GenBank Nucleotide #: NM_001533
• UniProt Accession #: P14866
• Molecular Weight: 65kDa
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein L isoform a
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein L
• NCBI Official Symbol: HNRNPL
• NCBI Official Synonym Symbols: HNRPL; hnRNP-L; P/OKcl.14
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein L
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein L
• UniProt Gene Name: HNRNPL
• UniProt Synonym Gene Names: HNRPL; hnRNP L
• UniProt Entry Name: HNRPL_HUMAN

NCBI Description

Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP L: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; RNA splicing; gene expression