Immunofluorescence (IF)
Rabbit EGR1 Polyclonal Antibody | anti-EGR1 antibody
Anti EGR1 polyclonal antibody
Reactivity
HumanOther species were not tested
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
EGR1; Polyclonal Antibody; Anti EGR1 polyclonal antibody; Early growth response protein 1; EGR-1; AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24; KROX24; ZNF225; anti-EGR1 antibody
Host
Rabbit
Reactivity
Human
Other species were not tested
Other species were not tested
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Form/Format
Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-EGR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:2000-1:8000
IHC: 1:200-1:400
IHC: 1:200-1:400
Immunogen
Recombinant protein of human EGR1 (Gln282-Ser433)
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 degree C for frequentUse. Store at -20 to -80 degree C for twelve months from the date of receipt.
Immunofluorescence (IF)
Immunofluorescence (IF)
Related Product Information for anti-EGR1 antibody
EGR1, also named as Early growth response protein 1, is a 543 amino acid protein, which belongs to the EGR C2H2-type zinc-finger protein family. EGR1 as a transcriptional regulator recognizes and binds to the DNA sequence 5'-GCG (T/G) GGGCG-3' (EGR-site) in the promoter region of target genes and binds double-stranded target DNA, irrespective of the cytosine methylation status. EGR1 regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. EGR1 activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation and plays a role in the regulation of cell survival, proliferation and cell death. Egr1 is suggested to be a 55-kDa protein according to the translation of its coding sequence. Analysis of cytoplasmic and nuclear fractions of normal and irradiated native CNS tissue by Western blotting revealed a 110-kDa band for Egr1 localized in the cytoplasm and a 75-kDa version for the nuclear Egr1 (PMID: 17497096).
Product Categories/Family for anti-EGR1 antibody
References
PubMed: 20121949; PubMed: 25258363; PubMed: 25999311
NCBI and Uniprot Product Information
Similar Products
Product Notes
The EGR1 (Catalog #AAA1560196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti EGR1 polyclonal antibody reacts with Human Other species were not tested and may cross-react with other species as described in the data sheet. AAA Biotech's EGR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:2000-1:8000 IHC: 1:200-1:400. Researchers should empirically determine the suitability of the EGR1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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