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Immunofluorescence (IF)

Rabbit Vimentin Polyclonal Antibody | anti-Vimentin antibody

Anti Vimentin polyclonal antibody

Reactivity
Human
Other species were not tested
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
Vimentin; Polyclonal Antibody; Anti Vimentin polyclonal antibody; CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; VIM; VIME; anti-Vimentin antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Other species were not tested
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Form/Format
Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-Vimentin antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:2000-1:8000
IHC: 1:200-1:400
Immunogen
Recombinant protein of human Vimentin (Met1-Glu466)
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 degree C for frequentUse. Store at -20 to -80 degree C for twelve months from the date of receipt.

Immunofluorescence (IF)

Immunofluorescence (IF)

Immunofluorescence (IF)

Immunofluorescence (IF)

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-Vimentin antibody
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA, 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49kDa and 60kDa) had also been reported.(PMID: 8640945, 22728585).
Product Categories/Family for anti-Vimentin antibody
References
PMID: 21746880; PMID: 21465480; PMID: 8601315

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,652 Da
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
Protein Family
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Uniprot Description

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30

Research Articles on Vimentin

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Product Notes

The Vimentin vim (Catalog #AAA1560155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti Vimentin polyclonal antibody reacts with Human Other species were not tested and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:2000-1:8000 IHC: 1:200-1:400. Researchers should empirically determine the suitability of the Vimentin vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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