Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.)

Rabbit Vinculin Polyclonal Antibody | anti-VCL antibody

Vinculin Antibody (biotin)

Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Human, Mouse, Rat, Rabbit, Chicken
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column.
Synonyms
Vinculin; Polyclonal Antibody; Vinculin Antibody (biotin); CMD1W; CMH15; Metavinculin; MVCL; vinculin; anti-VCL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Rabbit, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
Biotin-Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1066
Applicable Applications for anti-VCL antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Western blot at 0.5 - 1 mug/ml.
Conjugate
Biotin
Immunogen
Biotin-Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
Buffer
Biotin-Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
Species
Human
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.)

Western Blot (WB) (Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.)
Related Product Information for anti-VCL antibody
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Product Categories/Family for anti-VCL antibody
References
1. Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525. 2. Gilmore AP, Jackson P, Waites GT, et al. Further characterization of the talin-binding site in the cytoskeletal protein vinculin. J. Cell Sci. 1992; 103:719-31. 3. Deakin NO, Ballestrem C, and Turner CE. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS One 2012; 7:e37990. 4. Goldmann WH, Auernheimer V, Thievessen I, et al. Vinculin, cell mechanics and tumour cell invasion. Cell Biol. Int. 2013; Feb 1.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 117 kDa
Observed: 117 kDa
NCBI Official Full Name
vinculin isoform VCL
NCBI Official Synonym Full Names
vinculin
NCBI Official Symbol
VCL
NCBI Official Synonym Symbols
MV; MVCL; CMD1W; CMH15; HEL114
NCBI Protein Information
vinculin
UniProt Protein Name
Vinculin
Protein Family
UniProt Gene Name
VCL
UniProt Synonym Gene Names
MV

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: adherens junction; cell-cell adherens junction; costamere; cytoskeleton; cytosol; extracellular region; focal adhesion; intercellular junction; protein complex

Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; ubiquitin protein ligase binding

Biological Process: apical junction assembly; cell adhesion; cell motility; cell-matrix adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; neutrophil degranulation; platelet degranulation

Disease: Cardiomyopathy, Dilated, 1w

Research Articles on VCL

Similar Products

Product Notes

The VCL vcl (Catalog #AAA154475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vinculin Antibody (biotin) reacts with Human, Mouse, Rat, Rabbit, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Vinculin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Western blot at 0.5 - 1 mug/ml. Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vinculin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.