Rabbit VCL Antibody | anti-VCL antibody

VCL Antibody

Cat. Num. AAA8502751

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: VCL; VCL Antibody; MV; MVCL; CMD1W; CMH15; anti-VCL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1066

• Applicable Applications for anti-VCL antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human VCL
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of various celllines, using VCL antibody.)

Related Product Information for anti-VCL antibody

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4). Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1). In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2). Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-alpha and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5, 6). This change in vinculin allows the binding of a number of other proteins, including talin, alpha-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2, 4). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7, 8). This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9).

Product Categories/Family for anti-VCL antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Izard, T. et al. (2004) Nature 427, 171-5. 2. Humphries, J.D. et al. (2007) J Cell Biol 179, 1043-57. 3. Witt, S. et al. (2004) J Biol Chem 279, 31533-43. 4. Xu, W. et al. (1998) Development 125, 327-37. 5. Ziegler, W.H. et al. (2002) J Biol Chem 277, 7396-404. 6. Zhang, Z. et al. (2004) Mol Biol Cell 15, 4234-47. 7. Rodríguez Fernández, J.L. et al. (1993) J Cell Biol 122, 1285-94. 8. Samuels, M. et al. (1993) J Cell Biol 121, 909-21. 9. Yang, H.J. et al. (2010) Cancer Invest 28, 127-34.

NCBI and Uniprot Product Information

• NCBI GI #: 4507877
• NCBI GeneID: 7414
• NCBI Accession #: NP_003364.1
• NCBI GenBank Nucleotide #: NM_003373.3
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7
• Molecular Weight: 124kDa
• NCBI Official Full Name: vinculin isoform VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin
• UniProt Protein Name: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.