Rabbit VTI1b Polyclonal Antibody | anti-VTI1b antibody

VTI1b Antibody

Cat. Num. AAA154372

• Gene Names: VTI1B; VTI1; VTI2; VTI1L; v-SNARE; vti1-rp1; VTI1-LIKE
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: VTI1b; Polyclonal Antibody; VTI1b Antibody; Vesicle transport through interaction with t-SNAREs 1B; VTI1; VTI1-LIKE; VTI1L; VTI2; v-SNARE; vti1-rp1; anti-VTI1b antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist; this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 232

• Applicable Applications for anti-VTI1b antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Immunogen: VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: HeLa Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of VTI1b in HeLa cell lysate with VTI1b antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of VTI1b in human brain tissue with VTI1b antibody at 5 μg/mL.)

Related Product Information for anti-VTI1b antibody

Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4).

Product Categories/Family for anti-VTI1b antibody

Autophagy

References

Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30. Kreykenbohm V, Wenzel D, Antonin W et al. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. Eur. J. Cell Biol. 2002; 81:273-80. Antonin W, Riedel D, von Mollard GF, et al. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. J. Neurosci. 2000; 20:5724-32. Furuta N, Fujita N, Noda T, et al. Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes. Mol. Biol. Cell. 2010; 21:1001-10.

NCBI and Uniprot Product Information

• NCBI GI #: 5454166
• NCBI GeneID: 10490
• NCBI Accession #: NP_006361
• NCBI GenBank Nucleotide #: NM_006370.2
• UniProt Accession #: Q9UEU0; O43547; Q96J28
• Molecular Weight: Predicted: 14, 19, 26 kDa; Observed: 27 kDa
• NCBI Official Full Name: vesicle transport through interaction with t-SNAREs homolog 1B
• NCBI Official Synonym Full Names: vesicle transport through interaction with t-SNAREs 1B
• NCBI Official Symbol: VTI1B
• NCBI Official Synonym Symbols: VTI1; VTI2; VTI1L; v-SNARE; vti1-rp1; VTI1-LIKE
• NCBI Protein Information: vesicle transport through interaction with t-SNAREs homolog 1B
• UniProt Protein Name: Vesicle transport through interaction with t-SNAREs homolog 1B
• Protein Family: Vesicle transport through interaction with t-SNAREs
• UniProt Gene Name: VTI1B
• UniProt Synonym Gene Names: VTI1; VTI1L; VTI1L1; VTI2
• UniProt Entry Name: VTI1B_HUMAN

Uniprot Description

VTI1B: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence. Belongs to the VTI1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum membrane; Golgi apparatus; integral to membrane; intracellular membrane-bound organelle; late endosome membrane; lysosomal membrane; perinuclear region of cytoplasm; recycling endosome; SNARE complex; synaptic vesicle; vesicle

Molecular Function: chloride channel inhibitor activity; protein binding; SNAP receptor activity; SNARE binding

Biological Process: cell proliferation; ER to Golgi vesicle-mediated transport; Golgi to vacuole transport; intra-Golgi vesicle-mediated transport; protein targeting to vacuole; retrograde transport, endosome to Golgi; vesicle docking during exocytosis; vesicle fusion with Golgi apparatus; vesicle-mediated transport