Western Blot (WB)
(Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.)
Rabbit IQSEC1 Polyclonal Antibody | anti-IQSEC1 antibody
IQSEC1 Antibody
Gene Names
IQSEC1; BRAG2; GEP100; ARFGEP100; ARF-GEP100
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
IQSEC1 antibody is affinity chromatography purified via peptide column.
Synonyms
IQSEC1; Polyclonal Antibody; IQSEC1 Antibody; IQ motif and Sec7 domain 1; ADP-ribosylation factors guanine nucleotide-exchange protein 100; ARFGEP100; BRAG2; Brefeldin-resistant Arf-GEF 2 protein; anti-IQSEC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.
Purity/Purification
IQSEC1 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
963
Applicable Applications for anti-IQSEC1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 mug/ml.
Conjugate
Unconjugated
Immunogen
IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1
Buffer
IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IQSEC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.)
Western Blot (WB)
(Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.)
Immunocytochemistry (IHC)
(Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.Red: IQSEC1 Antibody (8009)Blue: DAPI staining)
Immunofluorescence (IF)
(Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.Red: IQSEC1 Antibody (8009)Blue: DAPI staining)
Related Product Information for anti-IQSEC1 antibody
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 106 kDa
Observed: 103 kDa
Observed: 103 kDa
NCBI Official Full Name
IQ motif and SEC7 domain-containing protein 1 isoform b
NCBI Official Synonym Full Names
IQ motif and Sec7 domain 1
NCBI Official Symbol
IQSEC1
NCBI Official Synonym Symbols
BRAG2; GEP100; ARFGEP100; ARF-GEP100
NCBI Protein Information
IQ motif and SEC7 domain-containing protein 1; brefeldin A-resistant ARF-GEF2; brefeldin-resistant Arf-GEF 2 protein; ADP-ribosylation factors guanine nucleotide-exchange protein 2; ADP-ribosylation factors guanine nucleotide-exchange protein 100
UniProt Protein Name
IQ motif and SEC7 domain-containing protein 1
Protein Family
UniProt Gene Name
IQSEC1
UniProt Synonym Gene Names
ARFGEP100; BRAG2; KIAA0763
UniProt Entry Name
IQEC1_HUMAN
Uniprot Description
IQSEC1: In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. Belongs to the BRAG family. Interacts with ARF6. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF
Chromosomal Location of Human Ortholog: 3p25.2
Cellular Component: membrane; trans-Golgi network; nucleus
Molecular Function: ARF guanyl-nucleotide exchange factor activity
Biological Process: vesicle-mediated transport; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity
Research Articles on IQSEC1
Similar Products
Product Notes
The IQSEC1 iqsec1 (Catalog #AAA153501) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IQSEC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IQSEC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 mug/ml. Researchers should empirically determine the suitability of the IQSEC1 iqsec1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IQSEC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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