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Western Blot (WB) (Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.)

Rabbit SRSF3 Polyclonal Antibody | anti-SRSF3 antibody

SRSF3 Antibody

Gene Names
SRSF3; SFRS3; SRp20
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
SRSF3 antibody is affinity chromatography purified via peptide column.
Synonyms
SRSF3; Polyclonal Antibody; SRSF3 Antibody; Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; SFRS3; Splicing factor arginine/serine-rich 3; SRP20; serine/arginine-rich splicing factor 3; anti-SRSF3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SRSF3 antibody is human, mouse and rat reactive.
Purity/Purification
SRSF3 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
164
Applicable Applications for anti-SRSF3 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 mug/ml.
Conjugate
Unconjugated
Immunogen
SRSF3 antibody was raised against a 19 amino acid peptide near the center of human SRSF3.
Buffer
SRSF3 antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SRSF3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.)

Western Blot (WB) (Western blot analysis of SRSF3 in K562 cell lysate with SRSF3 antibody at (A) 1 and (B) 2 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL .)

Immunocytochemistry (IHC) (Immunocytochemistry of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL .)

Immunofluorescence (IF)

(Immunofluorescence of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL.Red: SRSF3 Antibody (8179)Blue: DAPI staining)

Immunofluorescence (IF) (Immunofluorescence of SRSF3 in K562 cells with SRSF3 antibody at 2 μg/mL.Red: SRSF3 Antibody (8179)Blue: DAPI staining)
Related Product Information for anti-SRSF3 antibody
SRSF3, a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome, may be involved in RNA processing in relation with cellular proliferation and/or maturation (1,2). Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins (3). The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation (3,4).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 18 kDa

Observed: 19 kDa
NCBI Official Full Name
serine/arginine-rich splicing factor 3
NCBI Official Synonym Full Names
serine/arginine-rich splicing factor 3
NCBI Official Symbol
SRSF3
NCBI Official Synonym Symbols
SFRS3; SRp20
NCBI Protein Information
serine/arginine-rich splicing factor 3; pre-mRNA-splicing factor SRP20; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD
UniProt Protein Name
Serine/arginine-rich splicing factor 3
UniProt Gene Name
SRSF3
UniProt Synonym Gene Names
SFRS3; SRP20
UniProt Entry Name
SRSF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

SFRS3: May be involved in RNA processing in relation with cellular proliferation and/or maturation. Belongs to the splicing factor SR family.

Protein type: RNA-binding; RNA processing; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription

Research Articles on SRSF3

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Product Notes

The SRSF3 srsf3 (Catalog #AAA153478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SRSF3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SRSF3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). SRSF3 antibody can be used for detection of SRSF3 by Western blot at 1 - 2 mug/ml. Researchers should empirically determine the suitability of the SRSF3 srsf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SRSF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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