Rabbit TNFSF4 Polyclonal Antibody | anti-TNFSF4 antibody

TNFSF4 Antibody

Cat. Num. AAA153379

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TNFSF4 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TNFSF4; Polyclonal Antibody; TNFSF4 Antibody; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40L; tumor necrosis factor (ligand) superfamily; member 4; anti-TNFSF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: TNFSF4 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; TNFSF4 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 183

• Applicable Applications for anti-TNFSF4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
• Application Notes: TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 ?g/mL.; Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples.; All other applications and species not yet tested.
• Homology: Predicted species reactivity based on immunogen sequence: Rabbit: (91%
• Immunogen: Rabbit polyclonal TNFSF4 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TNFSF4.; The immunogen is located within the last 50 amino acids of TNFSF4.
• Positive Control: 1) Cat. No. MBS151642 - Rat Spleen Tissue Lysate; 2) Cat. No. MBS154333 - Human Spleen Tissue Slide
• Preparation and Storage: TNFSF4 antibody can be stored at 4 degree C for three months and -20 degree C.

Western Blot (WB)

(Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mL)

Immunohistochemistry (IHC)

(Immunohistochemistry of TNFSF4 in human spleen tissue with TNFSF4 antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of TNFSF4 in human spleen tissue with TNFSF4 antibody at 20 μg/ml.)

Related Product Information for anti-TNFSF4 antibody

TNFSF4 Antibody: The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317
• NCBI GenBank Nucleotide #: NM_003326.4
• UniProt Accession #: P23510; Q5JZA5; Q8IV74; Q9HCN9
• Molecular Weight: 20 kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4; CD134 ligand; glycoprotein Gp34; OX40 antigen ligand; tax-transcriptionally activated glycoprotein 1 (34kD)
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: extracellular space; cell surface; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: negative regulation of T-helper 1 cell differentiation; positive regulation of interleukin-12 production; negative regulation of cytokine secretion; positive regulation of T cell cytokine production; defense response to nematode; positive regulation of activated T cell proliferation; signal transduction; positive regulation of interleukin-10 production; positive regulation of interleukin-4 production; T cell proliferation; positive regulation of T-helper 2 cell differentiation; regulation of adaptive immune response; negative regulation of regulatory T cell differentiation; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of memory T cell differentiation; cholesterol metabolic process; positive regulation of T-helper 2 type immune response; response to virus; negative regulation of transcription factor activity; positive regulation of immunoglobulin mediated immune response; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of interleukin-2 production; positive regulation of alpha-beta T cell proliferation; immune response; negative regulation of transcription, DNA-dependent; acute inflammatory response; positive regulation of inflammatory response

Disease: Myocardial Infarction, Susceptibility To