Western Blot (WB)
(Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL. )
Rabbit RUSC1 Polyclonal Antibody | anti-RUSC1 antibody
RUSC1 Antibody
Gene Names
RUSC1; NESCA
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
RUSC1 Antibody is affinity chromatography purified via peptide column.
Synonyms
RUSC1; Polyclonal Antibody; RUSC1 Antibody; NESCA; RUN and SH3 domain-containing protein 1; New molecule containing SH3 at the carboxy-terminus; Nesca; RUN and SH3 domain containing 1; anti-RUSC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
RUSC1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
902
Applicable Applications for anti-RUSC1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 mug/mL.
Conjugate
Unconjugated
Immunogen
RUSC1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RUSC1.
Buffer
RUSC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
RUSC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL. )
Western Blot (WB)
(Western blot analysis of RUSC1 in A-20 cell lysate with RUSC1 antibody at 1 μg/mL. )
Related Product Information for anti-RUSC1 antibody
RUSC1 Antibody: RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells. RUSC1 has also been shown to interact with IκB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-κB activation in many signaling pathways such as TNF-R or the TLR pathways. RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination. Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-κB activation pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,216 Da
NCBI Official Full Name
RUN and SH3 domain-containing protein 1 isoform a
NCBI Official Synonym Full Names
RUN and SH3 domain containing 1
NCBI Official Symbol
RUSC1
NCBI Official Synonym Symbols
NESCA
NCBI Protein Information
RUN and SH3 domain-containing protein 1; new molecule containing SH3 at the carboxy-terminus
UniProt Protein Name
RUN and SH3 domain-containing protein 1
Protein Family
UniProt Gene Name
RUSC1
UniProt Synonym Gene Names
NESCA; Nesca
UniProt Entry Name
RUSC1_HUMAN
Uniprot Description
RUSC1: Putative signaling adapter which may play a role in neuronal differentiation. May be involved in regulation of NGF- dependent neurite outgrowth. Proposed to play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins. Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: microtubule cytoskeleton; Golgi apparatus; postsynaptic membrane; microtubule; postsynaptic density; cytoplasmic membrane-bound vesicle; early endosome; cytoplasm; cytoplasmic vesicle; cell junction; nucleus
Molecular Function: protein binding; SH3/SH2 adaptor activity; actin binding
Biological Process: protein polyubiquitination; positive regulation of signal transduction
Research Articles on RUSC1
Similar Products
Product Notes
The RUSC1 rusc1 (Catalog #AAA151353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RUSC1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RUSC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). RUSC1 antibody can be used for detection of RUSC1 by Western blot at 1 mug/mL. Researchers should empirically determine the suitability of the RUSC1 rusc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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