Rabbit anti-Human ILPIP Polyclonal Antibody | anti-STRADB antibody

ILPIP Antibody

Cat. Num. AAA151181

• Gene Names: STRADB; PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: ILPIP Antibody is affinity chromatography purified via peptide column.
• Synonyms: ILPIP; Polyclonal Antibody; ILPIP Antibody; PAPK; ILPIPA; ALS2CR2; CALS-21; PRO1038; STE20-related kinase adapter protein beta; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein; STRAD beta; STE20-related kinase adaptor beta; anti-STRADB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Anti-ILPIP is specific for human ILPIP only.
• Purity/Purification: ILPIP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-STRADB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ILPIP antibody can be used for the detection of ILPIP by Western blot at 0.5 to 2 mug/mL. Aa 34 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ILPIP antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human ILPIP.
• Buffer: ILPIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ILPIP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ILPIP in human heart lysate with ILPIP antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively. )

Immunohistochemistry (IHC)

(Immunohistochemistry of ILPIP in human heart tissue with ILPIP antibody at 5 μg/mL.)

Related Product Information for anti-STRADB antibody

ILPIP Antibody: ILPIP (ILP-interacting protein) has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family. Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1β converting enzyme of Fas-induced apotosis. This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival, suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

NCBI and Uniprot Product Information

• NCBI GI #: 13027388
• NCBI GeneID: 55437
• NCBI Accession #: NP_061041
• NCBI GenBank Nucleotide #: NM_018571.5
• UniProt Accession #: Q9C0K7; Q5BKY7; Q9P1L0
• Molecular Weight: 31,219 Da
• NCBI Official Full Name: STE20-related kinase adapter protein beta isoform 1
• NCBI Official Synonym Full Names: STE20-related kinase adaptor beta
• NCBI Official Symbol: STRADB
• NCBI Official Synonym Symbols: PAPK; ILPIP; ILPIPA; ALS2CR2; CALS-21; PRO1038
• NCBI Protein Information: STE20-related kinase adapter protein beta; STRAD beta; pseudokinase ALS2CR2; ILP-interacting protein ILPIPA; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
• UniProt Protein Name: STE20-related kinase adapter protein beta
• Protein Family: STE20-related kinase adapter protein
• UniProt Gene Name: STRADB
• UniProt Synonym Gene Names: ALS2CR2; ILPIP; STRAD beta
• UniProt Entry Name: STRAB_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

STLK6: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; STLK subfamily

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: cell morphogenesis; insulin receptor signaling pathway; activation of protein kinase activity; JNK cascade; mitotic cell cycle; protein export from nucleus; cell cycle arrest; protein amino acid phosphorylation