Western Blot (WB)
(Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human, Mouse APC2 Polyclonal Antibody | anti-APC2 antibody
APC2 Antibody
Gene Names
APC2; APCL
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
APC2 Antibody is affinity chromatography purified via peptide column.
Synonyms
APC2; Polyclonal Antibody; APC2 Antibody; APCL; Adenomatous polyposis coli protein 2; Adenomatous polyposis coli protein-like; APC-like; adenomatosis polyposis coli 2; anti-APC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
APC2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
2303
Applicable Applications for anti-APC2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2.
Buffer
APC2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
APC2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.)
Related Product Information for anti-APC2 antibody
APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
243,821 Da
NCBI Official Full Name
APCL protein
NCBI Official Synonym Full Names
adenomatosis polyposis coli 2
NCBI Official Symbol
APC2
NCBI Official Synonym Symbols
APCL
NCBI Protein Information
adenomatous polyposis coli protein 2; adenomatous polyposis coli like
UniProt Protein Name
Adenomatous polyposis coli protein 2
Protein Family
UniProt Gene Name
APC2
UniProt Synonym Gene Names
APCL; APC-like
UniProt Entry Name
APC2_HUMAN
Uniprot Description
APCL: Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling. Belongs to the adenomatous polyposis coli (APC) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: microtubule cytoskeleton; Golgi membrane; Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasm; actin filament; catenin complex
Molecular Function: protein binding; microtubule binding; beta-catenin binding
Biological Process: Wnt receptor signaling pathway
Research Articles on APC2
Similar Products
Product Notes
The APC2 apc2 (Catalog #AAA150831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APC2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's APC2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the APC2 apc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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