Rabbit anti-Human, Mouse TTBK1 Polyclonal Antibody | anti-TTBK1 antibody

TTBK1 Antibody

Cat. Num. AAA150715

• Gene Names: TTBK1; BDTK
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TTBK1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TTBK1; Polyclonal Antibody; TTBK1 Antibody; BDTK; KIAA1855; Tau-tubulin kinase 1; Brain-derived tau kinase; tau tubulin kinase 1; anti-TTBK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TTBK1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1321

• Applicable Applications for anti-TTBK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TTBK1 antibody can be used for detection of TTBK1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TTBK1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TTBK1.
• Buffer: TTBK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TTBK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TTBK1 in Jurkat lysate with TTBK1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TTBK1 in mouse brain tissue with TTBK1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TTBK1 in Human Brain tissue with TTBK1 antibody at 20 μg/mL.)

Related Product Information for anti-TTBK1 antibody

TTBK1 Antibody: Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients. Multiple isoforms of TTBK1 are known to exist. This antibody is predicted to not cross-react with TTBK2.

NCBI and Uniprot Product Information

• NCBI GI #: 97203020
• NCBI GeneID: 84630
• UniProt Accession #: Q5TCY1; Q2L6C6; Q6ZNH0; Q8N444; Q96JH2; A2A2U5
• Molecular Weight: 69,084 Da
• NCBI Official Full Name: Tau-tubulin kinase 1
• NCBI Official Synonym Full Names: tau tubulin kinase 1
• NCBI Official Symbol: TTBK1
• NCBI Official Synonym Symbols: BDTK
• NCBI Protein Information: tau-tubulin kinase 1; brain-derived tau kinase
• UniProt Protein Name: Tau-tubulin kinase 1
• Protein Family: Tau-tubulin kinase
• UniProt Gene Name: TTBK1
• UniProt Synonym Gene Names: BDTK; KIAA1855
• UniProt Entry Name: TTBK1_HUMAN

Uniprot Description

TTBK1: Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU. Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; CK1 group; TTBK family

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: regulation of cell shape; peptidyl-serine phosphorylation; substantia nigra development