Rabbit anti-Human CARD8 Polyclonal Antibody | anti-CARD8 antibody

CARD8 Antibody

Cat. Num. AAA150623

• Gene Names: CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: CARD8 Antibody is affinity chromatography purified via peptide column.
• Synonyms: CARD8; Polyclonal Antibody; CARD8 Antibody; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL; KIAA0955; Caspase recruitment domain-containing protein 8; Apoptotic protein NDPP1; caspase recruitment domain family; member 8; anti-CARD8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: CARD8 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 431

• Applicable Applications for anti-CARD8 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: CARD8 antibody can be used for detection of CARD8 by Western blot at 2 and 4 mug/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8.
• Buffer: CARD8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: CARD8 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 μg/mL.)

Related Product Information for anti-CARD8 antibody

CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.

NCBI and Uniprot Product Information

• NCBI GI #: 12247742
• NCBI GeneID: 22900
• UniProt Accession #: Q9Y2G2; Q6PGP8; Q96P82; B5KVR6; B7Z496; B7Z4A2; E9PEM7; G3XAM9
• Molecular Weight: 60,652 Da
• NCBI Official Full Name: caspase recruitment domain protein 8
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 8
• NCBI Official Symbol: CARD8
• NCBI Official Synonym Symbols: NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL
• NCBI Protein Information: caspase recruitment domain-containing protein 8; apoptotic protein NDPP1; CARD inhibitor of NF-kappaB-activating ligands; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
• UniProt Protein Name: Caspase recruitment domain-containing protein 8
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD8
• UniProt Synonym Gene Names: KIAA0955; NDPP1; CARDINAL; TUCAN
• UniProt Entry Name: CARD8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; NACHT domain binding; protein homodimerization activity; caspase activator activity

Biological Process: caspase activation; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion; negative regulation of I-kappaB kinase/NF-kappaB cascade