Mouse anti-Human CARD8 Monoclonal Antibody | anti-CARD8 antibody

CARD8 (Caspase Recruitment Domain, Cardinal/TUCAN)

Cat. Num. AAA600245

• Gene Names: CARD8; NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL; FLJ18119; FLJ18121; KIAA0955; MGC57162; DKFZp779L0366
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Dot Blot
• Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Synonyms: CARD8; Monoclonal Antibody; CARD8 (Caspase Recruitment Domain; Cardinal/TUCAN); Anti -CARD8 (Caspase Recruitment Domain; anti-CARD8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C55
• Specificity: Recognizes human CARD8.
• Purity/Purification: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
• Concentration: 0.1 mg/ml (varies by lot)

• Applicable Applications for anti-CARD8 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Dot Blot (DB)
• Application Notes: Recommended Dilutions:; Flow Cytometry: 0.5-2ug/ml; Western Blot: 0.2-2ug/ml; Immunocytochemistry: 2-100ug/ml; Immunoprecipitation: 100-500ug/sample; Optimal dilutions should be determined by the researcher.
• Grade: Affinity Purified
• Immunogen: Partial recombinant sequence from human CARD8
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of human CARD8 in HeLa cells whole cell lysate (50ug) using MBS600245 . Predicted molecular weight: 48kD)

Immunoprecipitation (IP)

(Immunoprecipitation analysis in RIPA lysate of HeLa cells using MBS600245)

Testing Data

(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS600245 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using MBS600245. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.)

Related Product Information for anti-CARD8 antibody

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARDcontaining protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo- ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-£eB activation.

Product Categories/Family for anti-CARD8 antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 7662404
• NCBI GeneID: 22900
• NCBI Accession #: NP_055774.1
• NCBI GenBank Nucleotide #: NM_014959.2
• UniProt Accession #: Q9Y2G2; Q6PGP8; Q96P82; B7Z496; Q6MZI8
• NCBI Official Full Name: caspase recruitment domain-containing protein 8
• NCBI Official Synonym Full Names: caspase recruitment domain family, member 8
• NCBI Official Symbol: CARD8
• NCBI Official Synonym Symbols: NDPP; DACAR; DAKAR; NDPP1; TUCAN; CARDINAL; FLJ18119; FLJ18121; KIAA0955; MGC57162; DKFZp779L0366
• NCBI Protein Information: caspase recruitment domain-containing protein 8; OTTHUMP00000228260; OTTHUMP00000228262; OTTHUMP00000228264; OTTHUMP00000228265; OTTHUMP00000228267; OTTHUMP00000228271; OTTHUMP00000228272; OTTHUMP00000228273; apoptotic protein NDPP1; CARD inhibitor of NF-kappaB-activating ligands; tumor up-regulated CARD-containing antagonist of CASP9; tumor up-regulated CARD-containing antagonist of caspase nine
• UniProt Protein Name: Caspase recruitment domain-containing protein 8
• Protein Family: Caspase recruitment domain-containing protein
• UniProt Gene Name: CARD8
• UniProt Synonym Gene Names: KIAA0955; NDPP1
• UniProt Entry Name: CARD8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq]

Uniprot Description

CARD8: Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; NACHT domain binding; protein homodimerization activity; caspase activator activity

Biological Process: caspase activation; positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion; negative regulation of I-kappaB kinase/NF-kappaB cascade