Western Blot (WB)
(Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.)
Rabbit VPS53 Polyclonal Antibody | anti-VPS53 antibody
VPS53 Antibody
Gene Names
VPS53; HCCS1; PCH2E; hVps53L; pp13624
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
VPS53 Antibody is affinity chromatography purified via peptide column.
Synonyms
VPS53; Polyclonal Antibody; VPS53 Antibody; HCCS1; hVps53L; pp13624; Vacuolar protein sorting-associated protein 53 homolog; vacuolar protein sorting 53 homolog (S. cerevisiae); anti-VPS53 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
VPS53 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
832
Applicable Applications for anti-VPS53 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 2.5 mug/mL.
Conjugate
Unconjugated
Immunogen
VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human VPS53.
Buffer
VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
VPS53 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of VPS53 in 293 cells with VPS53 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of VPS53 in 293 cells with VPS53 antibody at 2.5 μg/mL.)
Related Product Information for anti-VPS53 antibody
VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
94,405 Da
NCBI Official Full Name
vacuolar protein sorting 53 (yeast), isoform CRA_a
NCBI Official Synonym Full Names
vacuolar protein sorting 53 homolog (S. cerevisiae)
NCBI Official Symbol
VPS53
NCBI Official Synonym Symbols
HCCS1; PCH2E; hVps53L; pp13624
NCBI Protein Information
vacuolar protein sorting-associated protein 53 homolog
UniProt Protein Name
Vacuolar protein sorting-associated protein 53 homolog
Protein Family
UniProt Gene Name
VPS53
UniProt Entry Name
VPS53_HUMAN
NCBI Description
This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]
Uniprot Description
VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; endosome membrane
Molecular Function: protein binding
Biological Process: protein transport; retrograde transport, endosome to Golgi
Disease: Pontocerebellar Hypoplasia, Type 2e
Research Articles on VPS53
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Product Notes
The VPS53 vps53 (Catalog #AAA150579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS53 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VPS53 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 2.5 mug/mL. Researchers should empirically determine the suitability of the VPS53 vps53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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