Rabbit FEZ1 Polyclonal Antibody | anti-FEZ1 antibody

FEZ1 Antibody

Cat. Num. AAA150572

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: FEZ1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: FEZ1; Polyclonal Antibody; FEZ1 Antibody; Fasciculation and elongation protein zeta-1; Zygin I; fasciculation and elongation protein zeta 1 (zygin I); anti-FEZ1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: FEZ1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 392

• Applicable Applications for anti-FEZ1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: FEZ1 antibody can be used for the detection of FEZ1 by Western blot at 1 mug/mL. Despite its predicted molecular weight, FEZ1 will often run at higher molecular weight in SDS-PAGE.
• Conjugate: Unconjugated
• Immunogen: FEZ1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FEZ1.
• Buffer: FEZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: FEZ1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SAPAP2 in Raji cell lysate with SAPAP2 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-FEZ1 antibody

FEZ1 Antibody: Similar to its C. elegans homolog UNC-76, mammalian FEZ1, an abundant membrane protein and protein kinase C (PKC)-Z substrate, is involved in axonal guidance. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm. FEZ1 is then able to interact with a number of proteins involved in axonal outgrowth and cellular transport such as DISC1, a candidate gene for schizophrenia, as well as kinesin-1, a microtubule-based motor protein. FEZ1 also interacts with proteins involved in transcriptional regulation and chromatin remodeling, suggesting that FEZ1 may have nuclear regulatory functions in addition to those for neuronal development. At least two distinct isoforms of FEZ1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 13431526
• NCBI GeneID: 9638
• UniProt Accession #: Q99689; O00679; O00728; Q6IBI7
• Molecular Weight: 12,074 Da
• NCBI Official Full Name: Fasciculation and elongation protein zeta-1
• NCBI Official Synonym Full Names: fasciculation and elongation protein zeta 1 (zygin I)
• NCBI Official Symbol: FEZ1
• NCBI Protein Information: fasciculation and elongation protein zeta-1; zygin I; zygin-1
• UniProt Protein Name: Fasciculation and elongation protein zeta-1
• Protein Family: Fasciculation and elongation protein
• UniProt Gene Name: FEZ1
• UniProt Entry Name: FEZ1_HUMAN

NCBI Description

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

FEZ1: May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. Able to restore partial locomotion and axonal fasciculation to C.elegans unc-76 mutants in germline transformation experiments. May participate in the transport of mitochondria and other cargos along microtubules. Belongs to the zygin family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; mitochondrion; axon; dendrite; cytoplasm; plasma membrane

Molecular Function: protein binding; gamma-tubulin binding; protein kinase C binding; protein N-terminus binding

Biological Process: establishment of mitochondrion localization; axon guidance; nervous system development; transport; cell adhesion