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Western Blot (WB) (Western blotAll lanes: Peroxisomal bifunctional enzyme antibody at 2ug/ml+mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 80,70 kDaObserved band size: 79 kDa)

Rabbit anti-Human Peroxisomal bifunctional enzyme Polyclonal Antibody | anti-PBE antibody

Rabbit anti-human Peroxisomal bifunctional enzyme polyclonal Antibody

Gene Names
EHHADH; LBP; ECHD; LBFP; PBFE; FRTS3; L-PBE
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Synonyms
Peroxisomal bifunctional enzyme; Polyclonal Antibody; Rabbit anti-human Peroxisomal bifunctional enzyme polyclonal Antibody; PBFE; EHHADH; ECHD; anti-PBE antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Sequence Length
627
Applicable Applications for anti-PBE antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
IHC:1:20-1:200
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Peroxisomal bifunctional enzyme protein(1-723aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-99388 / sc-99386
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Peroxisomal bifunctional enzyme antibody at 2ug/ml+mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 80,70 kDaObserved band size: 79 kDa)

Western Blot (WB) (Western blotAll lanes: Peroxisomal bifunctional enzyme antibody at 2ug/ml+mouse kidney tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 80,70 kDaObserved band size: 79 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human rectum tissue using MBS1492690 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum tissue using MBS1492690 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1492690 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS1492690 at dilution 1:100)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,154 Da
NCBI Official Full Name
peroxisomal bifunctional enzyme isoform 2
NCBI Official Synonym Full Names
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
NCBI Official Symbol
EHHADH
NCBI Official Synonym Symbols
LBP; ECHD; LBFP; PBFE; FRTS3; L-PBE
NCBI Protein Information
peroxisomal bifunctional enzyme
UniProt Protein Name
Peroxisomal bifunctional enzyme
UniProt Gene Name
EHHADH
UniProt Synonym Gene Names
ECHD; PBE; PBFE
UniProt Entry Name
ECHP_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

EHHADH: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid; Mitochondrial; Carbohydrate Metabolism - propanoate; Isomerase; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 4.2.1.17; Lyase; EC 1.1.1.35; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - lysine degradation; Oxidoreductase; EC 5.3.3.8

Chromosomal Location of Human Ortholog: 3q26.3-q28

Cellular Component: cytosol; mitochondrion; peroxisome

Molecular Function: 3-hydroxyacyl-CoA dehydrogenase activity; dodecenoyl-CoA delta-isomerase activity; enoyl-CoA hydratase activity; enzyme binding; protein binding; receptor binding

Biological Process: fatty acid beta-oxidation; internal protein amino acid acetylation

Disease: Fanconi Renotubular Syndrome 3

Research Articles on PBE

Similar Products

Product Notes

The PBE ehhadh (Catalog #AAA1492690) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Peroxisomal bifunctional enzyme polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxisomal bifunctional enzyme can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the PBE ehhadh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxisomal bifunctional enzyme, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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