Rabbit anti-Human NAD-dependent protein deacetylase sirtuin-3 Polyclonal Antibody | anti-SIRT3 antibody

Rabbit anti-human NAD-dependent protein deacetylase sirtuin-3, mitochondrial polyclonal Antibody(SIRT3)

Cat. Num. AAA1492283

• Gene Names: SIRT3; SIR2L3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: NAD-dependent protein deacetylase sirtuin-3; Polyclonal Antibody; Rabbit anti-human NAD-dependent protein deacetylase sirtuin-3; mitochondrial polyclonal Antibody(SIRT3); hSIRT3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIRT3; SIR2L3; anti-SIRT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 257

• Applicable Applications for anti-SIRT3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:200-1000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human NAD-dependent protein deacetylase sirtuin-3, mitochondrial protein (20-130aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49744 / sc-49743 / sc-99143
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody at 1ug/ml+HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,29 kDaObserved band size: 44 kDa)

Western Blot (WB)

(Western BlotPositive WB detected in: hepG2 cellAll lanes: SIRT3 antibody at 0.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,29 kDaObserved band size: 44 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human breast cancer using MBS1492283 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1492283 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human pancreas using MBS1492283 at dilution 1:100)

Related Product Information for anti-SIRT3 antibody

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

NCBI and Uniprot Product Information

• NCBI GI #: 63054862
• NCBI GeneID: 23410
• NCBI Accession #: NP_001017524.1
• NCBI GenBank Nucleotide #: NM_001017524.2
• UniProt Accession #: Q9NTG7; Q9Y6E8; B7Z5U6
• Molecular Weight: 28,567 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b
• NCBI Official Synonym Full Names: sirtuin 3
• NCBI Official Symbol: SIRT3
• NCBI Official Synonym Symbols: SIR2L3
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-3, mitochondrial
• Protein Family: NAD-dependent protein deacetylase sirtuin
• UniProt Gene Name: SIRT3
• UniProt Synonym Gene Names: SIR2L3; hSIRT3
• UniProt Entry Name: SIR3_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT3: a soluble mitochondrial NAD-dependent protein deacetylase. Can deacetylate and thereby activate a central metabolic regulator in the mitochondrial matrix, glutamate dehydrogenase. Mitochondrial proteins are hyperacetylated in SIRT3-deficient mice, but not in SIRT4 or SIRT5 deficient mice. SIRT3-deficient mice have no obvious metabolic phenotype, and the process of adaptive thermogenesis is normal. Can deacetylate and activate isocitrate dehydrogenase 2, an enzyme that participates in regulation of the citric acid cycle and the regeneration of antioxidants. Inhibited by nicotinamide, but not by trichostatin A. Its unprocessed form is enzymatically inactive. It is processed by mitochondrial processing peptidase (MPP) in the mitochondrial matrix to give an enzymatically active 28 kDa product.

Protein type: EC 3.5.1.-; Mitochondrial; Deacetylase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: mitochondrial inner membrane; mitochondrial matrix; mitochondrion

Molecular Function: NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; zinc ion binding

Biological Process: aerobic respiration; mitochondrion organization and biogenesis; organelle organization and biogenesis; protein amino acid ADP-ribosylation; protein amino acid deacetylation