DNA endonuclease RBBP8 (RBBP8) Recombinant Protein | RBBP8 recombinant protein

Recombinant Human DNA endonuclease RBBP8 (RBBP8) , partial

• Gene Names: RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA endonuclease RBBP8 (RBBP8); Recombinant Human DNA endonuclease RBBP8 (RBBP8); partial; RBBP8 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 867

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for RBBP8 recombinant protein

This protein is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and
or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 4506441
• NCBI GeneID: 5932
• NCBI Accession #: NP_002885.1
• NCBI GenBank Nucleotide #: NM_002894.2
• UniProt Accession #: Q99708; O75371; Q8NHQ3; A6NKN2; A8K8W6; E7ETY1
• Molecular Weight: 98,434 Da
• NCBI Official Full Name: DNA endonuclease RBBP8 isoform a
• NCBI Official Synonym Full Names: RB binding protein 8, endonuclease
• NCBI Official Symbol: RBBP8
• NCBI Official Synonym Symbols: RIM; COM1; CTIP; JWDS; SAE2; SCKL2
• NCBI Protein Information: DNA endonuclease RBBP8
• UniProt Protein Name: DNA endonuclease RBBP8
• Protein Family: DNA endonuclease
• UniProt Gene Name: RBBP8
• UniProt Synonym Gene Names: CTIP; CtIP; RBBP-8; RIM; SAE2

NCBI Description

The protein encoded by this gene is a ubiquitously expressed nuclear protein. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. This protein complexes with transcriptional co-repressor CTBP. It is also associated with BRCA1 and is thought to modulate the functions of BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. It is suggested that this gene may itself be a tumor suppressor acting in the same pathway as BRCA1. Three transcript variants encoding two different isoforms have been found for this gene. More transcript variants exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in DNA-end resection, the first step of double-strand break (DSB) repair through the homologous recombination (HR) pathway. HR is restricted to S and G2 phases of the cell cycle and preferentially repairs DSBs resulting from replication fork collapse. Key determinant of DSB repair pathway choice, as it commits cells to HR by preventing classical non-homologous end-joining (NHEJ). Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage (PubMed:10764811, PubMed:10910365, PubMed:15485915, PubMed:16581787, PubMed:16818604, PubMed:17965729, PubMed:19202191, PubMed:19759395, PubMed:20064462, PubMed:20829486). During immunoglobulin heavy chain class-switch recombination, promotes microhomology-mediated alternative end joining (A-NHEJ) and plays an essential role in chromosomal translocations ().