E3 ubiquitin-protein ligase RNF146 (Rnf146) Recombinant Protein | Rnf146 recombinant protein

Recombinant Rat E3 ubiquitin-protein ligase RNF146 (Rnf146)

Cat. Num. AAA1482845

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: E3 ubiquitin-protein ligase RNF146 (Rnf146); Recombinant Rat E3 ubiquitin-protein ligase RNF146 (Rnf146); Rnf146 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-352, full length protein
• Sequence: MAGCGEIDHSLNMLPTNKKASETCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCVKGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYVWYYEGRNGWWQYDERTSRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDSNTVNLARESSADGADSGSAHTGASVQLPVPSSTRPLTSVDGQLTSPVTPSPDAGASLEDSFAHLQLSGDSIAERSHRGEGEEDHESPSSGRVPDTSTEETESDASSDIEDAPVVVAQHSLTQQRLLVSSANQTVAERSDRPVAGGGTMSVNVRSRRPDGQCTVTEV
• Sequence Length: 352

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 158854035
• NCBI GeneID: 308051
• NCBI Accession #: NP_001012060.2
• NCBI GenBank Nucleotide #: NM_001012060.2
• UniProt Accession #: Q5XIK5
• Molecular Weight: 38,224 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF146
• NCBI Official Synonym Full Names: ring finger protein 146
• NCBI Official Symbol: Rnf146
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF146
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF146
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf146

Uniprot Description

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation ().