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Western Blot (WB) (Western blot analysis of Beta actin expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Beta actin Antibody | anti-ACTB antibody

Beta actin antibody

Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified
Synonyms
Beta actin; Beta actin antibody; ACTIN; BETA-ACTIN; beta actin; actin beta; anti-ACTB antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
Beta actin antibody detects endogenous levels of total Beta actin
Purity/Purification
Immunogen affinity purified
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
375
Applicable Applications for anti-ACTB antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:10000
Immunogen
A synthesized peptide derived from human Beta actin
Preparation and Storage
Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of Beta actin expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Western Blot (WB) (Western blot analysis of Beta actin expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-ACTB antibody
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta-and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
60
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43kDa
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
NCBI Protein Information
actin, cytoplasmic 1
UniProt Protein Name
Actin, cytoplasmic 1
Protein Family
UniProt Gene Name
ACTB

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]

Uniprot Description

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.CautionWas originally thought to be part of the MLL5-L complex, at least composed of KMT2E, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT (PubMed:19377461). However, the corresponding article has been retracted (PubMed:24336203).

Research Articles on ACTB

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Product Notes

The ACTB actb (Catalog #AAA8512517) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Beta actin antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beta actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:10000. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta actin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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