Tyrosine Kinase ErbB-4 Recombinant Protein | ErbB4 recombinant protein

Recombinant Human Tyrosine Kinase ErbB-4

Cat. Num. AAA147366

• Gene Names: ERBB4; HER4; p180erbB4
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Tyrosine Kinase ErbB-4; Recombinant Human Tyrosine Kinase ErbB-4; Tyrosine Kinase ErbB-4 Human Recombinant; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; 4ICD; E4ICD; s80HER4; ERBB4; HER4; receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1; receptor tyrosine-protein kinase erbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; proto-oncogene-like protein c-ErbB-4; ALS19; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4; human epidermal growth factor receptor 4; ErbB4 Human; ErbB4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The ErbB4 solution (0.25mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: QSVCAGTENK LSSLSDLEQQ YRALRKYYEN CEVVMGNLEI TSIEHNRDLS FLRSVREVTG YVLVALNQFR YLPLENLRII RGTKLYEDRY ALAIFLNYRK DGNFGLQELG LKNLTEILNG GVYVDQNKFL CYADTIHWQD IVRNPWPSNL TLVSTNGSSG CGRCHKSCTG RCWGPTENHC QTLTRTVCAE QCDGRCYGPY VSDCCHRECA GGCSGPKDTD CFACMNFNDS GACVTQCPQT FVYNPTTFQL EHNFNAKYTY GAFCVKKCpHNFVVDSSSCV RACPSSKMEV EENGIKMCKP CTDICPKACD GIGTGSLMSA QTVDSSNIDK FINCTKINGN LIFLVTGIHG DPYNAIEAID PEKLNVFRTV REITGFLNIQ SWPPNMTDFS VFSNLVTIGG RVLYSGLSLL ILKQQGITSL QFQSLKEISA GNIYITDNSN LCYYHTINWT TLFSTINQRI VIRDNRKAEN CTAEGMVCNH LCSSDGCWGP GPDQCLSCRR FSRGRICIES CNLYDGEFRE FENGSICVEC DPQCEKMEDG LLTCHGPGPD NCTKCSHFKD GPNCVEKCPD GLQGANSFIF KYADPDRECH PCHPNCTQGC NGPTSHDCIY YPWTGHSTLP QHARLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH

• Biological Activity: Measured by its binding ability in a functional ELISA with Human NRG1(CAT# MBS147326). The ED50 range <= 0.2ug/ml.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for ErbB4 recombinant protein

Introduction: Tyrosine Kinase ErbB-4 (ErbB4) is a receptor tyrosine kinase that is a member of the epidermal growth factor receptor family whose members play a role as receptors for the EGF family of growth factors. ErbB4is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains,PDZ domain binding motif, a tyrosine kinase domain and a phosphotidylinositol-3 kinase binding site. ErbB4binds its ligands with low affinity and is expressed in normal skeletal muscle, heart, pituitary, brain and some breast carcinomas. Monomeric ErbB4 takes part in neuronal development and development of the heart and cancer.
Description: ErbB4 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 863 amino acids (26-649 a.a) and having a molecular mass of 96.6kDa.
ErbB4 is fused to a 239 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques.

Product Categories/Family for ErbB4 recombinant protein

PROTEIN KINASES

NCBI and Uniprot Product Information

• NCBI GI #: 110825958
• NCBI GeneID: 2066
• NCBI Accession #: NP_001036064.1
• NCBI GenBank Nucleotide #: NM_001042599.1
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 146,808 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-2
• NCBI Official Synonym Full Names: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: nucleoplasm; mitochondrion; basolateral plasma membrane; mitochondrial matrix; plasma membrane; extracellular region; integral to membrane; cytosol; nucleus; receptor complex

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; cell fate commitment; phosphoinositide-mediated signaling; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of protein amino acid phosphorylation; mitochondrial fragmentation during apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Amyotrophic Lateral Sclerosis 19