EDAR recombinant protein

EDAR, 27-187aa, Human, hIgG-His-tag, Baculovirus

Cat. Num. AAA206808

• Gene Names: EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: EDAR; 27-187aa; Human; hIgG-His-tag; Baculovirus; Tumor necrosis factor receptor superfamily member EDAR; DL; ECTD10A; ECTD10B; ED1R; ED3; ED5; EDA-A1R; EDA1R; EDA3; HRM1; EDAR recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ADPEYSNCGE NEYYNQTTGL CQECPPCGPG EEPYLSCGYG TKDEDYGCVP CPAEKFSKGG YQICRRHKDC EGFFRATVLT PGDMENDAEC GPCLPGYYML ENRPRNIYGM VCYSCLLAPP NTKECVGATS GASANFPGTS GSSTLSPFQH AHKELSGQGH LATAAAAFES RACSLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH
• Sequence Length: 448

• Applicable Applications for EDAR recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: hIgG-His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for EDAR recombinant protein

EDAR, also known as tumor necrosis factor receptor superfamily member EDAR, is a single-pass type 1 transmembrane protein which is a member of TNF receptor superfamily. This protein was expressed reiteratively in signaling centers regulating key steps in morphogenesis. Also, it is a cell surface receptor for ectodysplasin A which plays an important role in the development of ectodermal tissues such as the skin. Also, defects in EDAR are a cause of ectodermal dysplasia anhidrotic (EDA), also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structure. Recombinant human EDAR, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for EDAR recombinant protein

Signal Transduction

References

Kumar A., et al., (2000) J. Biol. Chem. 276:2668-2677. Schneider P, et al., (2001) J. Biol. Chem. 276:18819-18827.

NCBI and Uniprot Product Information

• NCBI GI #: 11641231
• NCBI GeneID: 10913
• NCBI Accession #: NP_071731
• NCBI GenBank Nucleotide #: NM_022336.3
• UniProt Accession #: Q9UNE0; Q52LL5; Q9UND9; B2R9H2; B4DLC5; D3DX74; E9PC98
• Molecular Weight: 45.6kDa (413aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member EDAR
• NCBI Official Synonym Full Names: ectodysplasin A receptor
• NCBI Official Symbol: EDAR
• NCBI Official Synonym Symbols: DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
• NCBI Protein Information: tumor necrosis factor receptor superfamily member EDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member EDAR
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: EDAR
• UniProt Synonym Gene Names: DL

NCBI Description

This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for EDA isoform A1, but not for EDA isoform A2. Mediates the activation of NF-kappa-B and JNK. May promote caspase-independent cell death.