ATPase Inhibitory Factor 1 Recombinant Protein | ATPIF1 recombinant protein

Recombinant Human ATPase Inhibitory Factor 1

Cat. Num. AAA144384

• Gene Names: ATPIF1; IP; ATPI; ATPIP
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Synonyms: ATPase Inhibitory Factor 1; Recombinant Human ATPase Inhibitory Factor 1; ATPIF1 Human; ATPase Inhibitory Factor 1 Human Recombinant; ATPase inhibitor mitochondrial; Inhibitor of F(1)F(o)-ATPase; IF(1); IF1; ATPIF1; ATPI; IP; ATPIP; ATPIF1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE.
• Form/Format: ATPIF1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGSDQS ENVDRGAGSI REAGGAFGKR EQAEEERYFR AQSREQLAAL KKHHEEEIVH HKKEIERLQK EIERHKQKIK MLKHDD.
• Sequence Length: 106

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for ATPIF1 recombinant protein

Description: ATPIF1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 106 amino acids (26-106 a.a) and having a molecular mass of 12.2kDa.ATPIF1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: ATPase inhibitory factor 1 (ATPIF1) attaches to the C-terminal region of a beta subunit of the F1-ATPase at low pH values and, through interference of the beta and gamma subunit interaction, ATPIF1 controls the activity of the F1 (the hydrophilic catalytic core), F0 (the membrane embedded protein channel) ATPase. ATPIF1 overexpression in a number of human carcinomas additionally reinforces its participation in oncogenesis and offers insight into the transformed metabolism of cancer cells, including the reprogramming of energy metabolism in relation to glycolysis. Endogenous F1F(o)-ATPase inhibitor curbing ATP depletion when the mitochondrial membrane potential drops below a threshold and the F1F(o)-ATP synthase begins hydrolyzing ATP to pump protons out of the mitochondrial matrix.

Product Categories/Family for ATPIF1 recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 7705927
• NCBI GeneID: 93974
• NCBI Accession #: NP_057395.1
• NCBI GenBank Nucleotide #: NM_016311.4
• UniProt Accession #: Q9UII2; Q5JXL8; Q6IAQ7; Q9BSL9
• Molecular Weight: 6,592 Da
• NCBI Official Full Name: ATPase inhibitor, mitochondrial isoform 1
• NCBI Official Synonym Full Names: ATPase inhibitory factor 1
• NCBI Official Symbol: ATPIF1
• NCBI Official Synonym Symbols: IP; ATPI; ATPIP
• NCBI Protein Information: ATPase inhibitor, mitochondrial; ATP synthase inhibitor protein; ATPase inhibitor protein; IF(1); IF1; inhibitor of F(1)F(o)-ATPase
• UniProt Protein Name: ATPase inhibitor, mitochondrial
• Protein Family: ATPase inhibitor
• UniProt Gene Name: ATPIF1
• UniProt Synonym Gene Names: ATPI; IF(1); IF1
• UniProt Entry Name: ATIF1_HUMAN

NCBI Description

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor; Mitochondrial

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: cell surface; mitochondrion; mitochondrial proton-transporting ATP synthase complex

Molecular Function: calmodulin binding; enzyme inhibitor activity; angiostatin binding; protein homodimerization activity; ATPase inhibitor activity; ATPase binding

Biological Process: generation of precursor metabolites and energy; regulation of gene expression; negative regulation of hydrolase activity; negative regulation of endothelial cell proliferation; erythrocyte differentiation; angiogenesis; mitochondrial depolarization; protein homooligomerization; negative regulation of nucleotide metabolic process; heme biosynthetic process; protein homotetramerization