Dimethylarginine Dimethylaminohydrolase 1 Recombinant Protein | DDAH1 recombinant protein

Recombinant Human Dimethylarginine Dimethylaminohydrolase 1

Cat. Num. AAA144288

• Gene Names: DDAH1; DDAH; HEL-S-16
• Purity: Greater than 95% as determined by SDS-PAGE.
• Synonyms: Dimethylarginine Dimethylaminohydrolase 1; Recombinant Human Dimethylarginine Dimethylaminohydrolase 1; DDAH1 Human; Dimethylarginine Dimethylaminohydrolase 1 Human Recombinant; DDAH; DDAH-1; Dimethylargininase-1; dimethylargininase-1; DDAH1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% as determined by SDS-PAGE.
• Form/Format: The DDAH1 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0) 1mM DTT, 50mM NaCl and 10% glycerol.; Sterile Filtered clear solution.
• Concentration: 20ug/20ul (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS
• Sequence Length: 182

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for DDAH1 recombinant protein

Description: DDAH1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 308 amino acids (1-285a.a.) and having a molecular mass of 33.5kDa.DDAH1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: Dimethylarginine dimethylaminohydrolase 1, is a part of the Dimethylarginine Dimethylaminohydrolase gene family. DDAH1 participates in nitric oxide generation by regulating cellular concentrations of methylarginines, that in turn inhibit nitric oxide synthase activity. Deficiency of DDAH1 results in ADMA (asymmetric dimethylarginine) increase and a decrease in cGMP generation.

Product Categories/Family for DDAH1 recombinant protein

Enzymes; Hydrolase

NCBI and Uniprot Product Information

• NCBI GI #: 197313763
• NCBI GeneID: 23576
• NCBI Accession #: NP_001127917.1
• NCBI GenBank Nucleotide #: NM_001134445.1
• UniProt Accession #: O94760; Q5HYC8; Q86XK5
• Molecular Weight: 20,189 Da
• NCBI Official Full Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 2
• NCBI Official Synonym Full Names: dimethylarginine dimethylaminohydrolase 1
• NCBI Official Symbol: DDAH1
• NCBI Official Synonym Symbols: DDAH; HEL-S-16
• NCBI Protein Information: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH-1; DDAHI; NG, NG-dimethylarginine dimethylaminohydrolase; dimethylargininase-1; epididymis secretory protein Li 16
• UniProt Protein Name: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
• Protein Family: N(G),N(G)-dimethylarginine dimethylaminohydrolase
• UniProt Gene Name: DDAH1
• UniProt Synonym Gene Names: DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1
• UniProt Entry Name: DDAH1_HUMAN

NCBI Description

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]

Uniprot Description

DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.18; Hydrolase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: mitochondrion

Molecular Function: amino acid binding; dimethylargininase activity; metal ion binding; catalytic activity

Biological Process: positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; citrulline metabolic process; regulation of systemic arterial blood pressure; arginine catabolic process; nitric oxide mediated signal transduction