Phosphatase 3 Recombinant Protein | PPP3CA recombinant protein

Recombinant Human Protein Phosphatase 3, Catalytic subunit, Alpha Isozyme

Cat. Num. AAA144187

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Phosphatase 3; Recombinant Human Protein Phosphatase 3; Catalytic subunit; Alpha Isozyme; PPP3CA Human; Protein Phosphatase 3; Alpha Isozyme Human Recombinant; Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; PPP3CA; CALNA; CAN; CALN; CCN1; CNA1; PPP2B; CALNA1; PPP3CA recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The PPP3CA solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ
• Sequence Length: 521

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PPP3CA recombinant protein

Description: PPP3CA Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 534 amino acids (1-511 a.a.) and having a molecular mass of 60kDa. The PPP3CA is purified by proprietary chromatographic techniques.

Introduction: PPP3CA (aka Calcineurin A) is a major soluble calmodulin binding protein in the brain and a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively limited substrate specificity. PPP3CA activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2.

Product Categories/Family for PPP3CA recombinant protein

Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 6715568
• NCBI GeneID: 5530
• NCBI Accession #: NP_000935.1
• NCBI GenBank Nucleotide #: NM_000944.4
• UniProt Accession #: Q08209; Q8TAW9; A1A441; A8K3B7; A8W6Z7; A8W6Z8; B5BUA2
• Molecular Weight: 51,245 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 3, catalytic subunit, alpha isozyme
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; CAM-PRP catalytic subunit; calcineurin A alpha; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; calcineurin complex; cytosol; Z disc; sarcolemma

Molecular Function: protein dimerization activity; calmodulin binding; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; drug binding; protein serine/threonine phosphatase activity; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; transition between fast and slow fiber; calcineurin-NFAT signaling pathway; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle