Interleukin-21 Active Protein | mIL 21 active protein

Recombinant Mouse Interleukin-21

Cat. Num. AAA143602

• Gene Names: Il21; IL-21
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Interleukin-21; Recombinant Mouse Interleukin-21; IL 21 Mouse; Interleukin-21 Mouse Recombinant; IL-21; Il21; mIL 21 active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser
• Sequence Length: 146

• Solubility: It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells.
• Preparation and Storage: Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse IL21 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mIL 21 active protein

Description: Interleukin-21 Mouse Recombinant produced in E Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques.

Introduction: IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (degree C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.

Product Categories/Family for mIL 21 active protein

Cytokines and Growth Factors; Cytokines; Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 11140821
• NCBI GeneID: 60505
• NCBI Accession #: NP_068554.1
• NCBI GenBank Nucleotide #: NM_021782.3
• UniProt Accession #: Q9ES17
• Molecular Weight: 16,811 Da
• NCBI Official Full Name: interleukin-21 isoform 2
• NCBI Official Synonym Full Names: interleukin 21
• NCBI Official Symbol: Il21
• NCBI Official Synonym Symbols: IL-21
• NCBI Protein Information: interleukin-21
• UniProt Protein Name: Interleukin-21
• Protein Family: Interleukin
• UniProt Gene Name: Il21
• UniProt Synonym Gene Names: IL-21
• UniProt Entry Name: IL21_MOUSE

Uniprot Description

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Secreted; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process