Macrophage Inflammatory protein-3 alpha Active Protein | mMIP 3alpha active protein

Recombinant Mouse Macrophage Inflammatory protein-3 alpha (CCL20)

Cat. Num. AAA143277

• Gene Names: Ccl20; CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Macrophage Inflammatory protein-3 alpha; Recombinant Mouse Macrophage Inflammatory protein-3 alpha (CCL20); MIP 3a Mouse; Macrophage Inflammatory Protein-3 alpha Mouse Recombinant (CCL20); mMIP 3a; Small inducible cytokine A20 precursor; CCL20; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Liver and activation- regulated chemokine; CC chemokine LARC; Beta chemokine exodus-1; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; C-C motif chemokine 20; CC chemokine ST38; exodus-1; mMIP 3alpha active protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM
• Sequence Length: 96

• Solubility: It is recommended to reconstitute the lyophilized MIP-3a in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The Activity is calculated by its ability to chemoattract primary human T cells at 2-20ng/ml, corresponding to a specific activity of 50,000-500,000 units/mg.
• Preparation and Storage: Lyophilized MIP-3a although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CCL-20 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for mMIP 3alpha active protein

Description: Macrophage Inflammatory Protein-3 alpha Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.9 kDa. The MIP-3a is purified by proprietary chromatographic techniques.

Introduction: CCL20 is a chemotactic factor that draws lymphocytes & neutrophils, rather than monocytes. MIP-3 alpha inhibits proliferation of myeloid progenitors in colony formation assays. MIP3A plays a role in the formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. CCL20 holdes antibacterial activity e.coli atcc 25922 and s.aureus atcc 29213. CCL20 gene transcription is activated by H. pylori, which activates NF-kappaB through intracellular signal pathway which involves IkappaB kinase and NF-kappaB-inducing kinase. MIP-3 alpha is invloved in chemokine-mediated lymphocyte trafficking during gastric inflammation in Helicobacter infection. CCL20 expression is involved in the recruitment of CD45R0-positive T cell subsets into the intestinal lamina propria. MIP-3A is in charge of the advancement of pulpal inflammation through the recruitment of C-C motif Receptor 6-expressing lymphocytes Vaginal epithelial cells respond to factors present in semen by secreting MIP-3 alpha, which increases langerhans cells recruitment during HIV transmission.

Product Categories/Family for mMIP 3alpha active protein

Chemokines; MIP

NCBI and Uniprot Product Information

• NCBI GI #: 229092240
• NCBI GeneID: 20297
• NCBI Accession #: NP_001153210.1
• NCBI GenBank Nucleotide #: NM_001159738.1
• UniProt Accession #: O89093; Q9Z1X3
• Molecular Weight: 10,755 Da
• NCBI Official Full Name: C-C motif chemokine 20 isoform 2
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 20
• NCBI Official Symbol: Ccl20
• NCBI Official Synonym Symbols: CKb4; LARC; ST38; MIP3A; MIP-3A; Scya20; MIP-3[a]; exodus-1
• NCBI Protein Information: C-C motif chemokine 20; CC chemokine LARC; CC chemokine ST38; MIP-3-alpha; beta chemokine exodus-1; beta-chemokine exodus-1; liver and activation-regulated chemokine; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A20; small-inducible cytokine A20
• UniProt Protein Name: C-C motif chemokine 20
• UniProt Gene Name: Ccl20
• UniProt Synonym Gene Names: Larc; Scya20; MIP-3-alpha
• UniProt Entry Name: CCL20_MOUSE

Uniprot Description

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Chemokine

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; cytokine activity

Biological Process: immune response; positive regulation of interleukin-1 alpha biosynthetic process; chemotaxis; inflammatory response; positive regulation of nitric-oxide synthase biosynthetic process