Macrophage Inflammatory protein-1 beta Active Protein | mMIP 1b active protein
Recombinant Mouse Macrophage Inflammatory protein-1 beta (CCL4)
Gene Names
Ccl4; Act-2; Mip1b; Scya4; MIP-1B; AT744.1
Purity
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Macrophage Inflammatory protein-1 beta; Recombinant Mouse Macrophage Inflammatory protein-1 beta (CCL4); MIP 1b Mouse; Macrophage Inflammatory Protein-1 beta Mouse Recombinant (CCL4); Small inducible cytokine A4; CCL4; Macrophage inflammatory protein 1-beta; MIP-1- beta; MIP-1-beta(1-69); T-cell activation protein 2; ACT-2; PAT 744; H400; SIS-gamma; Lymphocyte activation gene 1 protein; LAG-1; HC21; G-26 T-lymphocyte-secreted protein; chemokine (C-C motif) ligand 4; ACT2; G-26; LAG1; MIP1B; SCYA2; SCYA4; AT744.1; MGC104418; MGC126025; MGC126026; mMIP 1b active protein
Host
E Coli
Purity/Purification
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence
APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA
Sequence Length
92
Solubility
It is recommended to reconstitute the lyophilized Mouse MIP-1b in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity
Mouse MIP-1 beta activity is calculated by the ability to chemoattract Human blood monocytes at 20-100ng/ml corresponding to a Specific Activity of 10,000-50,000IU/mg.
Preparation and Storage
Lyophilized Mouse MIP-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Mouse CCL4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for mMIP 1b active protein
Description: Macrophage Inflammatory Protein-1 beta Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7808 Dalton. The MIP-1b is purified by proprietary chromatographic techniques.
Introduction: Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1? and MIP-1? that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-? from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
Introduction: Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1? and MIP-1? that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophils and basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6 and TNF-? from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
Product Categories/Family for mMIP 1b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,168 Da
NCBI Official Full Name
C-C motif chemokine 4
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 4
NCBI Official Symbol
Ccl4
NCBI Official Synonym Symbols
Act-2; Mip1b; Scya4; MIP-1B; AT744.1
NCBI Protein Information
C-C motif chemokine 4; ACT2; MIP-1 beta; MIP-1-beta; SIS-gamma; immune activation protein 2; macrophage inflammatory protein 1-beta; small inducible cytokine A4; small-inducible cytokine A4
UniProt Protein Name
C-C motif chemokine 4
UniProt Gene Name
Ccl4
UniProt Synonym Gene Names
Mip1b; Scya4; ACT-2; ACT2; MIP-1-beta
UniProt Entry Name
CCL4_MOUSE
Uniprot Description
CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding
Biological Process: leukocyte chemotaxis; response to toxin; positive regulation of calcium-mediated signaling; immune response; positive regulation of calcium ion transport; positive regulation of tumor necrosis factor production; chemotaxis; inflammatory response; positive regulation of vascular permeability
Research Articles on mMIP 1b
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Product Notes
The mMIP 1b ccl4 (Catalog #AAA143275) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory protein-1 beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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